急性放射性脑损伤鼠模型的建立(英文)

Establishment of a murine model with acute radiative brain injury

背景:目前国内外关于放射性脑损伤动物模型的研究均处于探索中,尚未形成成熟的模型制作方法。目的:建立急性放射性脑损伤的鼠模型,为进一步研究放射性脑损伤正确有效的预防措施提供实验依据。设计:以实验动物为研究对象,随机对照观察研究。单位:一所大学医院动物实验室。材料:实验于2001-06/2002-08在中山大学附属第二医院实验室完成。SD大鼠60只,体质量(300±30)g,雌雄各半,空白对照组20只,实验组40只。方法:SD大鼠头部接受60Coγ射线照射,7Gy/次,1次/d,连续照射6d,总剂量42Gy。照射结束后每日观察大鼠摄食、饮水量及次数,自主活动情况,有无出现神经系统症状与体征,每周检查并记录大鼠头部照射区毛发与皮肤情况、体质量变化,照射结束后第3,7,14,30天断头取脑,行病理组织学检查。主要观察指标:①一般情况观察。②照射后脑组织病理改变。结果:照射第3天起即出现每日摄食、饮水量减少;照射第1,2天,自主活动较对照组增多,第3天起活动渐减少;无异常神经系统体征;随观察时间延长体质量增长较对照组慢,但差异无显著性意义;所有大鼠均于照射后约2周时出现照射野轻度脱毛;照射后出现脑组织神经元变性坏死。结论:该模型制作方法切实、可靠,较好地模拟放射性脑损伤的过程,可用于预防或减轻放射治疗对脑组织损伤的?

BACKGROUND:At present, the researches on murine model of acute radiation encephalopathy are still in investigation,and mature model making method is not clear. OBJECTIVE:To establish a murine model of acute radiation encephalopathy in order to provide a good foundation for further researches of radiation encephalopathy(REP) mechanism and therapy. DESIGN:Randomized controlled study based on the experimental animals. SETTING:Animal laboratory in a university hospital. The Second Affiliated Hospital of Sun Yat sen University. MATERIALS:The experiment was completed in the Laboratory of the Second Affiliated Hospital of Sun Yat sen University from June 2001 to August 2002.Totally 30 female and 30 male SD rats,weighted(300±30) g,were selected from the Animal Experiment Center of Sun Yat sen University,and randomly divided into blank control group with 20 rats and experimental group with 40 rats. METHODS:Fourty rats' brain received 60Co γ ray irradiation with the dosage of 7 Gy/time per day for 6 consecutive days with the total dosage of 42 Gy.The amount of ingestion and drinking,general activities,central nervous system(CNS) symptoms and signs were recorded every day.The hairs and skin of irradiated field and weight were checked and recorded weekly.On the 3rd,7th,14th,30th days after radiation,the brain tissue was collected and the histopathologic changes were examined. MAIN OUTCOME MEASURES:①Observation of general condition;②Histopathologic changes after radiation. RESULTS:Since the third day, the ingestion and drinking amount of irradiated rats were decreased.The general activities were increased for the first two days,but decreased without abnormal nervous signs on the 3rd day.The rats in experimental group had a slower weight gain than those of control group,and the difference between them was of no statistical significance.All rats had slight alopecia and neuronal necrosis 2 weeks after irradiation. CONCLUSION:The irradiation method is reliable,practical and good for modeling REP process,which can be used in preventing or reducing the harm effect of radiation therapy on brain tissue.