姜黄素对哮喘大鼠气道炎症与核因子κB表达的影响

Effect of curcumin on airway inflammation and the expression of nuclear factor kappa-B in rats with asthma

目的:探讨姜黄素对支气管大鼠气道炎症及核因子-κBNF-κB的影()响。方法:Wistar大鼠随机分为正常对照组、哮喘模型组、姜黄素组各12只,采用卵蛋白OVA等致敏大鼠哮喘模型,观察3组动物哮喘发作症()状、气道炎症情况及免疫组化染色后NF-κB的核着色情况。结果:哮喘模型组大鼠哮喘发作症状最明显,姜黄素组大鼠症状轻,正常对照组无症状。哮喘模型组支气管周围明显炎细胞浸润,且以浆细胞、嗜酸性粒细胞浸润为主,姜黄素组较哮喘模型组明显减轻,正常对照组无明显炎细胞浸润。肺泡灌洗液总细胞数及嗜酸性粒细胞哮喘模型组明显高于正常对照组和姜黄素组。肺组织石蜡切片免疫组化发现哮喘模型组NF-κB的核着色最强,姜黄素组较弱,正常对照组微弱表达。结论:姜黄素可抑制哮喘的慢性气道炎症,其机制可能与姜黄素抑制哮喘大鼠NF-κB的活性有关。

AIM: To investigate the influence of curcumin on airway inflammation and nuclear factor kappa B (NF κ B) in rats with asthma.METHODS: Wistar rats were randomly divided into three groups: normal control group (n=12), asthmatic model group (n=12) and curcumin treatment group (n=12).The asthmatic models were established by ovalbumin (OVA).The symptoms of asthmatic attack and airway inflammation as well as the NF κ B nuclear pigmenting after immohistochemical staining were observed.RESULTS: The symptoms of asthmatic attack was the most obvious in the asthmatic model group, but mild in the curcumin treatment group, and no symptom was observed in the normal control group. In the asthmatic model group, there were obvious infiltrations of inflammatory cells around the bronchi, and most of them were infiltrations of plasmacytes and eosinophils, which were remarkably relieved in the curcumin treatment group, and there was no obvious infiltration of inflammatory cells in the normal control group. The total cell amount in bronchoalveolar lavage fluid and eosinophil were obviously higher in the asthmatic model group than in the curcumin treatment group and normal control group. Lung tissue paraffin section immunohistochemistry showed that the nuclear pigmenting of NF κ B was the strongest in the asthmatic model group, weaker in the curcumin treatment group, and only slight expression in the normal control group.CONCLUSION: Curcumin can inhibit asthmatic chronic airway inflammation, and its mechamism may be correlated with the inhibition of curcumin to the activity of NF κ B in rats.