枳葛片对急性酒精中毒小鼠翻正反射及血液中各项生物化学参数的影响

Effect of zhihe pian on righting reflex and biochemical parameters in blood of mice with acute alcoholism

目的:探讨枳葛片对急性酒精(乙醇)中毒后小鼠的神经精神症状、血液中乙醇浓度和各项生物化学参数的影响。方法:实验于2003-06/09在解放军总医院生化科完成。将60只昆明小鼠随机分为6组,禁食12h后分别给蒸馏水6h和24h组对照组)、高剂(量枳葛片(6h组和24h组,1.5g/kg)、低剂量枳葛片(24h组,1g/kg)和海王金樽(24h对照药物,2.5g/kg),30min后全部实验动物按0.018mL/g体质量一次性灌胃给560mL/L乙醇,记录翻正反射消失和恢复时间,实验结束时取血测定血液中乙醇浓度和生化指标的变化。结果:枳葛片可明显延长小鼠翻正反射消失的时间(低剂量组t=-2.648,P<0.05,高剂量组t=-4.215,P<0.01),在24h组间比较结果提示枳葛片可缩短小鼠翻正反射的恢复时间且有量效关系(低剂量组t=2.224,P<0.05;高剂量组t=3.500,P<0.01)。血液检测结果提示枳葛片可逆转小鼠由于急性乙醇中毒所致的谷草转氨酶、乳酸脱氢酶、胆固醇和三酰甘油升高。与对照组比较,低剂量组中谷草转氨酶、乳酸脱氢酶、胆固醇含量差异有显著性意义(t=2.159～6.390,P<0.01或0.05),高剂量组中谷草转氨酶、乳酸脱氢酶、胆固醇、三酰甘油含量差异有显著性意义(t=2.750～5.597,P<0.01或0.05)。高剂量枳葛片可降低血液中的乙醇浓度(t=2.988,P<0.05)。

AIM:To investigate the effect of zhige pian(ZGP) on the neurological and psychiatric symptoms,concentration of alcohol and the biochemical parameters in blood of mice with acute alcoholism. METHODS:The experiment was done in the Department of Biochemistry,General Hospital of Chinese PLA during June and September 2003.After fasting for 12 hours,60 Kunming mice were divided into six groups,they were treated with distilled water(6 and 24 hour control groups),high dose of ZGP(1.5 g/kg for 6 and/or 24 hours),low dose of ZGP(1 g/kg for 24 hours) and King Drink(2.5 g/kg for 24 hours,medicine control group),respectively. After 30 minutes,all the animals were given gastric perfusion of alcohol(560 mL/L) according to 0.018 mL/10 g body mass.The dismissing and recovering times of righting reflex were recorded.At the end of the experiment,blood was drawn to determine the changes of alcohol concentration and the biochemical parameters. RESULTS:The dismissing time of righting reflex could be prolonged in ZGP groups of high dose(t=- 2.648,P< 0.05) and low dose(t=- 4.215,P< 0.01).Comparion among 24 hour groups showed that ZGP could shorten the recovering time of righting reflex dose dependently(low dose group:t =2.224,P< 0.05; high dose group:t=3.500,P< 0.01).Results of blood test indicated that the increases of glutamic oxaloacetic transaminase(GOT), lactate dehydrogenase(LDH),cholesterol and triglycerides(TG) caused by acute alcoholism could be reversed by ZGP.Compared with the control group,the contents of GOT,LDH, and cholesterol in the low dose ZGP group were significantly different(t=2.159 to 6.390,P< 0.01 or 0.05),the contents of GOT,LDH,cholesterol and TG were also significantly different in the high dose ZGP group(t=2.750 to 5.597,P< 0.01 or 0.05).High dose ZGP could decrease the concentration of alcohol in blood(t=2.988,P< 0.05). CONCLUSION:ZGP has the potential property of sober alcohol up and reduce the damage induced by alcoholism.