补肾中药复方改善骨质疏松模型大鼠皮质骨生物力学性能的实验(英文)

Amelioration of biomechanical property of cortical bone in osteoporotic model rats with the kidney tonifying compound of traditional Chinese medicine

背景:骨的生物力学性能优劣,取决于骨的结构和骨的材料。片面追求骨矿密度的提高,可能会引起骨生物力学性能的恶化。目前对骨质疏松治疗作用的不少研究过分强调了药物对骨矿密度的影响,对骨质疏松部位骨的整体生物力学性能的变化及其机制的研究还有待深入。设计:以实验动物为研究对象的完全随机对照的实验研究。目的:探讨补肾中药复方对去卵巢骨质疏松模型大鼠皮质骨(股骨)生物力学性能的作用及其相关的机制。单位:河南省洛阳正骨研究所生物医学工程实验室。材料:实验于2000-11/2001-06在河南省洛阳正骨研究所生物医学工程实验室完成。10月龄Wistar雌性大鼠50只,体质量(350±20)g。方法:将50只10月龄Wistar雌性大鼠随机分为5组:正常对照组、模型对照组、密骨胶囊组、仙灵骨葆组和倍美力组,每组10只。正常对照组做假手术,其余4组做卵巢切除术。术后正常饲养90d,第91天开始给药,连续用药90d,处死,测定股骨的骨矿密度、几何尺寸和股骨的生物力学性能。主要观察指标:①主要结局为股骨力学性能参数的比较。②次要结局为股骨几何结构参数、皮质骨面积和股骨各平面骨矿密度的变化。结果:模型大鼠股骨的生物力学性能明显下降,骨强度降低;股骨干外径变细,股骨中段骨皮质面积减少,骨矿密度有所下降。补肾中药复方?

BACKGROUND:The biomechanical property(BP) of the bone is decided by its geometric structure and component material. Merely pursuing increase of the bone mineral density(BMD) might lead to deterioration of bone BP. However at present,some researches on therapeutic action on osteoporosis emphasize excessively medical influence to BMD,and the change in the holistic BP of the bone in osteoporotic zone and its mechanism still need to investigate deeply.OBJECTIVE:To probe into the action and its mechanism of “ the kidney tonifying compound of the Traditional Chinese Medical (TCM) ” on BP of cortical bone in ovariectomized osteoporotic model rats.DESIGN:Completely randomized controlled experiment based on experimental animals. SETTING:Laboratory of Biomedical Engineering,Luoyang Hospital and Institute of Traditional Chinese Orthopedics and Traumatology in Henan Province.MATERIALS:The experiment was completed from November 2000 to July 2001 at Research Laboratory of Biomedical Engineering, Luoyang Institute of Traditional Chinese Orthopedics and Traumatology of Henan Province.The healthy Wistar female rats aged 10 months, weighing(350± 20) g.METHODS:Fifty Whistar female rats aged 10 months were randomly divided into 5 groups: the normal,model,premarin treated,xianling gubao treated and migu capsule treated with 10 in each group.The normal group was only given sham operation and the other four groups were ovariectomized.The rats after operation were fed normally for ninety days. Since the 91st day after operation, the rats had been given the medicines for 90 days and then killed. The thighbones were taken out, then BMD,femoral geometry sizes and BP were determined. MAIN OUTCOME MEASURES:① The primary sequel was the comparison of the parameters of femoral BP.② The secondary sequel was the changes in parameters of femoral geometric structure,area of cortical bone and BMD of every midsectional fomur. RESULTS:Femoral BP worsened significantly, its mechanical intensity reduced,its external diameter diminished, cortical bone area decreased and femoral BMD lowered in osteoporotic model rats. In comparison with the above,in “ the kidney tonifying compound of TCM ' groups(migu capsule group and xianling gubao group) femoral BP raised significantly,its mechanical intensity advanced,its external diameter augmented,cortical bone area aggrandized and femoral BMD enhanced. CONCLUSION:“ The kidney tonifying compound of TCM ' can improve BP of the cortical bone(thighbone) in ovariectomized osteoporosis rats. Its primary mechanism of action is that the TCM compound prescription could enhance“ the mechanism of biomechanical response and regulation'(MBRR) of macrostructure of cortical bone, consequently increase femoral external diameter, aggrandize cortical bone area and enhance BMD in ovariectomized rats.