组织工程化血管的生成及修复:大鼠血管内皮细胞增殖与碱性成纤维细胞生长因子的关系

Tissue engineered vascularization and vascular reconstruction:proliferation of vascular endothelial cells and basic fibroblast growth factor in rats

目的:观察碱性成纤维细胞生长因子(碱性成纤维细胞生长因子)对血管内皮细胞增殖的影响及其促细胞增殖作用与原癌基因c-fos和c-myc表达之间的关系。方法:实验于2002-06/2003-02在广东医学院附属医院整形外科研究所实验室完成。在建立血管内皮细胞体外培养模型的基础上,通过氚标记胸腺嘧啶脱氧核苷参入实验,Northern印迹分析,观察碱性成纤维细胞生长因子对血管内皮细胞DNA合成及原癌基因表达的影响。结果:碱性成纤维细胞生长因子作用血管内皮细胞后16h,血管内皮细胞氚标记胸腺嘧啶脱氧核苷参入率开始明显增加(P<0.05),24h达高峰(P<0.01);碱性成纤维细胞生长因子显著诱导原癌基因c-fos和c-myc表达,其表达活性分别于1h和2h达到高峰。结论:碱性成纤维细胞生长因子是血管内皮细胞的强效有丝分裂原,其对血管内皮细胞DNA合成的促进作用与原癌基因c-fos和c-myc表达明显相关。c-fos和c-myc原癌基因表达变化参与了碱性成纤维细胞生长因子促血管内皮细胞DNA合成过程,合理调控其表达变化有增强碱性成纤维细胞生长因子促血管内皮细胞增殖的生物学效应,为碱性成纤维细胞生长因子广泛而有效地应用于组织工程血管形成和血管移植及组织修复提供强有力的理论依据。

AIM:To investigate the effects of basic fibroblast growth factor(bFGF) on the proliferation of vascular endothelial cells(VECs) and the relationship of its ac celerating effect with the expression of protoonco gene c fos and c myc. METHODS:The experiment was done in the laboratory of Institute of Plastic Surg ery,Affiliated Hospital of Guangdong Medical College from June 2002 to February 2003.After the models of VECs cultured in vitro were established,the effects of bFGF on DNA synthesis of VECs and expression of c fos and c myc in VECs were d etermined by 3H TdR incorporation and Northern blot analysis. RESULTS:The value of 3H TdR incorporated into VECs significantly increased af ter VECs had been incubated with bFGF for 16 hours(P< 0.05),and reached its peak at 24 hours(P< 0.01). bFGF significantly induced the expression of protoonco g ene c fos and c myc.The maximal expression of c fos and c myc was respective ly at 1 hour and 2 hours after induced by bFGF. CONCLUSION:bFGF,a strong and effective mitogen for VECs,its accelerating effec t on DNA synthesis of VECs is obviously related to the expression of protoonco gene c fos and c myc induced by bFGF.The expression changes of c fos and c m yc are involved in bFGF promoting the DNA synthesis of VECs,so regulating their expression properly can enhance bFGF to accelerate the DNA synthesis of VECs,whi ch will provide strong theoretical bases for the application of bFGF in tissue e ngineered vascularization,blood vessel transplantation and vascular reconstructi on.