非特异性下腰痛患者血清中人巨细胞病毒pp65抗体检测与腰背痛的相关性

Detection of human cytomegalovirus pp65 antibody in serum of patients with nonsp ecial low back pain associated with low back pain

目的:通过对非特异性下腰痛患者血清中人巨细胞病毒pp65抗体的检测,试图探究人巨细胞病毒潜伏感染在非特异性下腰痛中的潜在致病作用。方法:实验于2002-05/2004-01在安徽医科大学第一附属医院康复医学科门诊完成。比较3组不同人群外周血清人巨细胞病毒pp65IgM抗体阳性水平。分别采集3组空腹静脉血2mL,作为待检血清。用间接酶联免疫吸附法即人巨细胞病毒pp65重组蛋白抗原包被96孔酶标板,依次加入待测血清。经酶标抗人抗体显色后,用酶标仪(BIO-RAD,model550,US-A)测定A值。结果以(样品A450-本底)÷(对照组A450-本底)≥2.1判为阳性。结果:非特异性下腰痛组人巨细胞病毒pp65IgM阳性率为48.21%(27/56),高于健康大学生组的17.86%(5/28),差异有显著性意义(χ2=7.294,P=0.007);非特异性下腰痛组的人巨细胞病毒pp65IgM阳性率高于脑瘫患儿组的26.42%(14/53),差异有显著性意义(χ2=5.514,P=0.019)。结论:非特异性下腰痛患者出现腰痛症状时可能与人巨细胞病毒的再激活有关,但需进一步确定人巨细胞病毒pp65抗体阳性水平与临床症状的相关性、是否需要相应干预和干预后的变化,以及进行相应组织细胞的病毒分离从而获得腰痛与人巨细胞病毒再激活感染病源学结论的直接证据。对人巨细胞病毒感染与非特异性下腰痛的相关性进?

AIM:To detect human cytomegalovirus(HCMV) pp65 antibody in serum of the patien ts with non special low back pain(NLBP) so as to probe into the potential etiol ogical effect of HCMV latent infection on NLBP. METHODS:This study was completed in outpatient department of Rehabilitation Me dicine in the Affiliated Hospital of Anhui Medical University between May 2002 a nd January 2004.The positive level of HCMV pp65 IgM antibody in peripheral serum was compared among 3 groups[NLBP,cerebral palsy(CP) and healthy undergraduate g roups].Under fasting,2 mL vein blood were collected from the three groups as ser um specimens.Indirect enzyme linked immunosorbent assay(ELISA) was used to coat 96 well enzyme labeled plate with HCMV pp65 recombinant antigen,then the serum specimens were added orderly.The A value was determined by enzyme labeled appa ratus(BIO RAD,model550,USA) after stained with enzyme labeled antibody.If A450 of Specimen minus background to A450 of Control minus background was more than or equal to 2.1,the result was regarded positive. RESULTS:The positive rate of HCMV pp65 IgM antibody in NLBP group was 48.21%( 27/56),which was not only higher than that in CP group(26.42%,14/53) with signi ficant difference(χ2=5.514,P=0.019),but also much higher than that in healthy u ndergraduate group(17.86%,5/28),also with significant difference(χ2=7.294,P=0. 007). CONCLUSION:HCMV reactivation is possibly related with NLBP especially when NLB P is symptomatic of lumbago symptom.However,other aspects must be studied furthe r such as the correlation between positive level of anti HCMVpp65 antibody and clinical symptom,and whether NLBP needs corresponding interference and its chang e after interference.It is the direct evidence of etiology between NLBP and HCMV reactivation infection that HCMV virus is separated from corresponding lumbar h istiocytes.The correlation between HCMV infection and NLBP has been studied cons tructively in our study,which provides new evidence for possible etiological fac tors,pathogenesis and clinical diagosis and treatment of NLBP.