瘢痕疙瘩组织中胰岛素样生长因子结合蛋白3的表达

Expression of insulin-like growth factor binding proteins-3 in keloid tissues

目的:胰岛素样生长因子-1瘢痕疙瘩的病理过程密切相关,胰岛素样生长因子结合蛋白3是与胰岛素样生长因子-1结合的调节蛋白,探讨胰岛素样生长因子结合蛋白3在瘢痕疙瘩形成中的作用。方法:实验于2004-04/07在长海医院中心实验室完成。18份被检测标本中包括瘢痕疙瘩6份,肥厚性瘢痕6份,成人正常皮肤组织6份。用免疫组织化学的方法和半定量反转录-聚合酶链反应的方法从蛋白和核酸两个水平检测胰岛素样生长因子结合蛋白3在不同的组织内的表达情况。结果:免疫组化结果显示,瘢痕疙瘩组胰岛素样生长因子结合蛋白3染色阳性面积明显大于正常皮肤组,差异有显著性意义(t=39.52,P<0.01)。反转录-聚合酶链反应结果显示胰岛素样生长因子结合蛋白3mRNA在瘢痕疙瘩中的表达明显高于正常皮肤标本,差异有显著性意义(t=18.19,P<0.01)。结论:胰岛素样生长因子结合蛋白3在瘢痕疙瘩组织中的表达明显增加,提示其参与了瘢痕疙瘩的形成过程。

AIM:Insulin like growth factor 1 is closely associated with the pathological formation of keloid.Insulin like growth factor binding protein 3(IGFBP 3) is the regulating protein bounded with insulin like growth factor 1.This paper aim s to investigate the role of IGFBP 3 in the pathogenesis of keloid. METHODS:The study was completed in the Central Laboratory of Changhai Hospital from April to July 2004.The expression of IGFBP 3 protein and mRNA in 6 keloid lesions,6 hypertrophic scars and 6 normal skin specimens was examined by using immunohistochemistry and semiquantitative reverse transcriptase polymerase chai n reaction(RT PCR) analysis. RESULTS:Immunohistochemical results demonstrated that the area of IGFBP 3 pos itive staining in keloid lesions was significantly larger than that in normal sk ins(t=39.52,P< 0.01).RT PCR analysis showed that the mean level of IGFBP 3 mRN A expression was significantly increased in keloid lesions,as compare with that in normal skins(t=18.19,P< 0.01). CONCLUSION:The increased expression of IGFBP 3 mRNA and protein in keloid les ions suggests that IGFBP 3 may play an important role in the pathogenesis of ke loid.