肺癌患者血清P53抗体和癌胚抗原检测结果比较

Outcomes of detection of the level of serum P53 antibodies versus carcinoembryonic antigen in patients with lung cancer

目的:观察不同病期、不同组织类型肺癌患者血清P53抗体、癌胚抗原水平单独与联合检测的结果,并比较其意义。方法:选择2000-01/2003-04在华北煤炭医学院附属开滦医院呼吸科、胸外科、肿瘤科住院的肺癌患者76例,男54例,女22例;年龄42～78岁。所有病例均确诊为肺癌,并同意参加此研究。鳞癌33例,腺癌23例,小细胞癌20例。早期肺癌23例,晚期肺癌53例。采用ELISA法检测肺癌患者血清P53抗体滴度,经酶联免疫检测仪测定吸光度值A450,计算P53抗体指数以判定血清P53抗体阴阳性。采用免疫分析仪检测癌胚抗原水平。阳性:P53抗体指数>0.428;癌胚抗原>10μg/L(正常值0～5μg/L)。阴性:P53抗体指数<0;癌胚抗原<0。结果:76例肺癌患者的血清P53抗体阳性率为49%(37/76)。单独检测:①早期肺癌患者血清P53抗体阳性率65%(15/23)明显高于癌胚抗原阳性率30%(7/23)(χ2=5.58,P<0.05);晚期肺癌P53抗体阳性率42%(22/53)与癌胚抗原阳性率28%(15/53)比较,差异无显著意义(P>0.05)。②鳞癌癌胚抗原阳性率15%(5/33)明显低于P53抗体阳性率48%(16/33)(χ2=8.45,P<0.01)。非小细胞癌癌胚抗原阳性率29%(16/56)明显低于P53抗体阳性率54%(30/56)(χ2=7.23,P<0.01)。联合检测:在早期肺癌中:P53抗体与癌胚抗原联合检测阳性率为78%(18/23)明显高于?

AIM:To observe the results of level detection of serum P53 antibodies,carcinoe mbryonic antigen(CEA) and combination of both in patients with lung cancer at di fferent stages or in different tissues,and compare significance of the above res ults. METHODS:Seventy six patients with lung carcinoma(54 males and 22 females,aged from 42 to 78 years) who had been finally diagnosed and agreed to attend the st udy were selected from the Department of Respiratory Medicine,Department of Onco logy and Department of Thoracic Surgery in Kailuan Hospital Affiliated to North China Coal Medical College between January 2000 and April 2003.Of the 76 cases o f lung carcinoma,33 were squamous carcinoma,23 adenocarcinoma and 20 small cell carcinoma;23 were at its early stage and 53 at its terminal stage.The titer of s erum P53 antibodies in lung carcinoma was detected with ELISA method,and A450 wi th enzyme linked immunoassay,followed by the calculation of P53 antibody indexe s to evaluate that they were negative or positive antibodies.The level of CEA wa s tested by immune analysis.Positive:the index of P53 antibodies more than 0.428 ;the level of CEA higher than 10 μg/L(0 to 5 μg/L is the normal level).Negativ e:the index of P53 antibodies less than 0;the level of CEA lower than 0. RESULTS:The positive rate of serum P53 antibodies in the 76 cases of lung carc inoma was 49%(37/76).Single detection for serum P53 antibodies or CEA:①In the early lung carcinoma patients,the positive rate of serum P53 antibodies was 65% (15/23),significantly higher than that of CEA(30%,7/23)(χ2=5.58,P< 0.05);But i n the terminal lung carcinoma patients,the former(42%,22/53) was insignificantl y different from the latter(28%,15/53)(P >0.05).②In the squamous carcinoma pat ients,the positive rate of CEA(15%,5/33) was significantly lower than that of s erum P53 antibodies(48%,16/33)(χ2=8.45,P< 0.01);Similarly,in the small cell ca rcinoma patients,the former(29%,16/56) was significantly lower than the latter( 54%,30/56)(χ2=7.23,P< 0.01).Combination detection for serum P53 antibodies and CEA:In the early lung carcinoma patients,the combination positive rate was 78(1 8/23),significantly higher than the positive rate of CEA(χ2=8.84,P< 0.01);In th e terminal lung carcinoma patients,the combination positive rate(53%,28/53) was significantly higher than the positive rate of CEA(χ2=6.61,P=0.01). CONCLUSION:The positive rate of serum P53 antibodies detected is obviously hig her than that of CEA;the combination positive rate of serum p53 antibodies and C EA is obviously higher than the positive rate of serum p53 antibodies or CEA.The positive serum P53 antibodies are related to adverse biological characteristics and bad prognosis of lung carcinoma.