增生性玻璃体视网膜病变中的增生膜变化:图像技术半定量胶原分析

Changes of periretinal membrane during proliferative vitreoretinopathy:a semiqua ntitative collagen analysis using imaging

目的:探讨增生性玻璃体视网膜病变的增生膜中Ⅰ和Ⅲ型胶原的含量和作用。方法:对22例视网膜脱离伴增生性玻璃体视网膜病变行玻璃体切除术时切除的增生膜组织用天狼猩红染色,偏振光观察,photoshop分析,半定量研究Ⅰ和Ⅲ型胶原的含量。结果:Ⅰ和Ⅲ型胶原在22例视网膜增生膜中均有表达,Ⅲ型胶原在D级膜中含量明显高于C级膜(15.41±1.07,5.36±0.45,t=3.58,P<0.05),但Ⅰ型胶原在D级膜和C级膜中含量基本相似(3.70±0.59,4.03±0.38,t=1.36,P>0.05)。结论:Ⅰ和Ⅲ型胶原的异常表达,作为细胞黏附支架,可促进细胞移动,变换细胞形态,促进细胞增生,促使增生性玻璃体视网膜病变不断发展,导致视网膜脱离。根据此原理在转录水平调节Ⅰ,Ⅲ型胶原表达,为阻断增生性玻璃体视网膜病变进展提供新的途径。

AIM:To investigate the expression and role of type Ⅰand Ⅲcollagen in periret inal membrane of proliferative vitreoretinopathy(PVR). METHODS:Periretinal membrane was obtained from 22 patients with retinal detach ment accompanied by PVR during vitrectomy.Then,the content of type Ⅰand Ⅲcolla gen was detected by sirius red staining,polarized light observation,Photoshop an alysis and semiquantitative study. RESULTS:The expression of type Ⅰand Ⅲcollagen was detected in all the perire tinal membranes.The expression of type Ⅲcollagen in D grade periretinal membra ne was significantly higher than that in C grade periretinal membrane(15.41±1. 07 vs 5.36±0.45,t=3.58,P< 0.05).But,the expression of type Ⅰcollagen was insig nificantly different between D grade periretinal membrane and C grade perireti nal membrane(3.70±0.59 vs 4.03±0.38,t=1.36,P >0.05). CONCLUSION:The expression of type Ⅰand Ⅲcollagen is abnormal in periretinal membrane.As the cell adhesion scaffold,they can accelerate cell locomotion,trans form cell shape,promote cell proliferation,alter the development of PVR and indu ce retinal detachment;Accordingly,regulating the expression of type Ⅰand Ⅲcoll agen in a transcription level provides a new approach to blocking the developmen t of PVR.