青光眼视功能损害中内皮素-1的作用及其机制

Role and mechanism of endothelin-1 in the deterioration of visual function of glaucomatous eyes

目的:为从分子生物学水平对青光眼的防治提供新的思路,探讨内皮素-1在青光眼患者视功能损害中的作用及作用机制。方法:实验于2002-07/2003-06在广东医学院附属医院中心实验室完成。根据视功能损害程度和沿盘面积比分为早期青光眼患者16例、进展期青光眼患者21例、晚期青光眼患者18例和正常志愿者30例。应用放射免疫法测定其血浆中内皮素-1的含量。结果:青光眼组,正常对照组,视功能损害早期,视功能损害进展期及视功能损害晚期血浆内皮素-1平均水平分别为(82.17±14.88),(65.35±18.45),(69.07±25.28),(91.23±13.26)及(107.06±34.02)mg/L。在视功能损害早期比正常对照组增加,但差异无显著性意义(t=3.98,P>0.01)。视功能损害进展期、晚期,均较正常对照组明显增加,差异有显著性意义(t=4.62,t=4.89,P<0.01);视功能损害进展期、晚期较损害早期明显增加,差异有显著性意义(t=4.37,t=4.53,P<0.01)。青光眼患者视功能损害早期、进展期、晚期沿盘面积比分别为0.43±0.11,0.76±0.23,0.94±0.21。沿盘面积比与内皮素-1的关系:r=0.9124,P<0.001,呈正相关。结论:青光眼患者血浆中内皮素-1的含量增加与其视功能损害程度有关。

AIM:To probe into the role and mechanism of ET 1 in the deterioration of visu al function in the patients with glaucoma in order to provide a new approach to the prevention and cure of glaucoma from molecular biology. METHODS:The experiment was completed in the central laboratory of the Affiliat ed Hospital of Guangdong Medical College from July 2002 to June 2003.According t o the deterioration degree of visual function and the area ratio of rim to disc, the ET 1 level in the plasma was determined by radioimmunoassay in 16 patients with early glaucoma,21 with glaucoma progressive glaucoma,18 with terminal glauc oma and 30 normal adults. RESULTS:The average ET 1 level in the plasma were (82.17±14.88),(65.35±18.4 5),(69.07±25.28),(91.23±13.26) and (107.06 ±34.02) mg/L respectively in glauc oma group,control group,the deterioration of visual function in early,progressiv e and terminal periods,and the level of ET 1 in deterioration of visual functio n in early period was higher than that in control group,but without significant difference(t=3.98,P >0.01).The level of ET 1 in the deterioration of visual fun ction in progressive or terminal period were either significantly increased as c ompared with that in the control group(t=4.62,t=4.89,P< 0.01) .The ET 1 level w as significantly increased in the deterioration of visual function in progressiv e and terminal period as compared with that in early period(t=4.37,t=4.53,P< 0.0 1).In the deterioration of visual function in early,progressive and terminal per iods,the average area ratio of rim to disc was 0.43±0.11,0.76±0.23,0.94±0.21. The area ratio of rim to disc and the level of ET 1 was positively correlated(r =0.912 4,P< 0.001). CONCLUSION:The increased ET 1 level in the plasma of glaucoma may be highly r elated to the deterioration degree of visual function in patients with glaucoma.