自体骨髓基质干细胞修复兔桡骨骨缺损的可行性(英文)

Feasibility of repairing bone defect of rabbit radius by autologous bone marrow stromal stem cells

背景:迄今为止临床上对较大范围骨缺损的修复仍未得到有效的解决。目的:探讨自体骨髓基质干细胞修复兔桡骨远端骨缺损的可行性。设计:以实验动物为研究对象,自身对照设计的探索性研究。单位:一所医科大学医院骨科。对象:实验于2002-10/2003-04在福建省医科所实验动物中心完成。健康新西兰大白兔12只,雌雄各半,体质量3.1～3.4kg。方法:取兔髂骨骨髓,体外分离培养扩增骨髓基质干细胞,与生物材料混合后植入体内修复兔桡骨远端骨缺损,应用X射线片、大体标本、组织切片观察修复效果。主要观察指标:主要结局:①大体标本及X射线片。②组织学观察。次要结局:骨髓基质干细胞分离与培养。结果:移植后12周,X射线片显示高密度新骨形成,大体标本上见白色皮质骨充满骨缺损处,组织切片显示骨缺损修复处骨小梁丰富,骨髓腔形成。结论:自体骨髓基质干细胞移植是修复兔桡骨远端骨缺损的理想方法。

BACKGROUND:There is no effective measure to repair the large scope bone defect in clinic till now. OBJECTIVE:To explore the feasibility on repairing bone defect of rabbit radius by autologous bone marrow stem cells. DESIGN:Self controlled groping study focused on experimental animals. SETTING:Department of orthopaedics of a university hospital. MATERIALS:The study was completed in the Experimental Animal Centre of Fujian Medical Science Institute.Twelve healthy New Zealand rabbits with half sex each were selected with body mass of 3.1 to 3.4 kg. METHODS:The marrow of rabbit ilium was collected and separated, cultured in vi tro to proliferate bone marrow stromal stem cells and then mixed with biological materials to transplant the distal bone defect area of rabbit radius.X ray,gro ss samples and histological section were used to observe the repairing effects. MAIN OUTCOME MEASURES:Main outcomes:①Gross samples and X ray;②Histological observation.Subordinate outcomes: isolation and culture of bone marrow stromal stem cells RESULTS:X ray showed that new bone with high density was formed 12 weeks afte r transplant and white cortical bone was seen in the defected area from gross sa mple while histological sections showed that there were plenty of bone trabecule s and the marrow cavity was formed. CONCLUSION:Transplant of autogenous bone marrow stromal stem cells is an ideal method to repair distal bone defect of rabbit radius.