地氟烷对全脑缺血再灌注损伤大鼠脑神经元及细胞骨架的可能性保护作用(英文)

Possibly protective effects of desflurane on the brain neurons and cellular skeleton in rats with global cerebral ischemia-reperfusion injury

背景:目前,形态学上地氟烷脑保护作用的证据尚不充足,机制还不清楚。目的:探讨地氟烷对全脑缺血再灌注损伤大鼠的脑保护作用及对凋亡和应激反应相关基因表达的影响。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所大学医院的麻醉科和神经外科。材料:实验于2003-02/2004-02在首都医科大学附属北京神经外科研究所完成。成年雄性Wistar大鼠17只,随机分为缺血组7只、地氟烷组7只和假手术对照组3只。干预:制备大鼠全脑缺血再灌注模型。地氟烷组再灌注开始后立即吸入地氟烷1h。缺血组和地氟烷组每组取3只大鼠于再灌注1h(假手术组于手术后1h)取脑,利用电镜技术观察皮质超微结构的变化。缺血组和地氟烷组其余4只大鼠,利用基因芯片结合图像分析技术检测凋亡和应激反应相关基因的差异表达情况。主要观察指标:①皮质超微结构变化。②基因芯片检测凋亡和应激反应相关基因的差异表达情况。结果:与缺血组比较,地氟烷组神经元固缩少,神经元的细胞器和微管结构基本正常。与缺血组比较,地氟烷组凋亡蛋白酶激活因子下调。结论:地氟烷对神经元、细胞骨架可能具有保护作用,其机制可能与凋亡蛋白酶激活因子下调有关。

BACKGROUND:At present,the proof of the cerebral protective effects of desflura ne on morphology is insufficient and the mechanism is unclear. OBJECTIVE:To investigate the cerebral protective effects of desflurane on comp lete cerebral ischemia reperfusion injury and its impacts on gene expression re lated with apoptosis and stress response. DESIGN:A randomized controlled trial based on the experimental animals. SETTING:Departments of anesthesiology and neurosurgery in a university hospita l. MATERIALS:The study was conducted in Beijing Neurosurgery Institute Affiliated with Capital University of Medical Sciences between February 2003 and February 2004.Seventeen adult male Wister rats were randomly divided into ischemia group( n=7),desflurane group (n=7) and sham operation group(n=3). INTERVENTIONS:Rat complete cerebral ischemia reperfusion model was establishe d.Desflurane was immediately inhaled for 1 hour after the beginning of reperfusi on in rats of desflurane group. Brains of were harvested from 3 rats of either i schemia group or desflurane group after 1 hour of reperfusion(after 1 hour of op eration in sham operation group) and the cortical ultrastructural changes were observed under electron microscope.The rest 4 rats of either ischemia group or d esflurane group were used for the analysis of the variable expression of genes r elated with apoptosis and stress response by gene chip combined image analysis t echnique. MAIN OUTCOME MEASURES:①cortical ultrastructural changes; ②variable expressio n of genes related with apoptosis and stress response analyzed by gene chip. RESULTS:Compared with ischemia group,desflurane group had less neuronal pyknos is with almost normal cell organs and ultrastructure in neurons.Compared with is chemia group, the apoptotic protease activating factor 1(APAF1) of desflurane gr oup down regulated. CONCLUSION:Desflurane might have protective effects on neurons and cell skelet on and its mechanism might be related with APAF1 down regulation.