应用Er：YAG激光消融兔角膜

Er:YAG Ablation of Cornea in Rabbits

新西兰白兔６只，分成１、２两组，每组３只（６眼）。在静脉麻醉下刮去角膜中部上皮，用Ｅｒ：ＹＡＧ激光１００～１４０ｍＪ、０．３２ｃｍ（能量密度为１２５０～１７５０ｍＪ／ｃｍ２）、１５０μｓ、１Ｈｚ，分别照射１组每眼６０次，２组１２０次。术后进行裂隙灯检查，并于术后３周、１月和４月摘下眼球，分别作光镜及扫描电镜检查。激光照射后即见到角膜表面出现黄白色凝固层。一般４～８天上皮修复完毕。术后３周至１月角膜基质混浊最明显，此时出现上皮过度增生，基质胶原纤维增殖，排列紊乱。术后４月角膜基本恢复透明。以上表现均以２组更为明显。本文并对Ｅｒ：ＹＡＧ激光进行角膜手术的可行性及优点进行了讨论。

Six New-Zealand white rabbits were divided into two groups,3 rabbits(6 eyes)in each group.The epithe-lium of the central area of the cornea was removed under veiious anesthesia,and each cornea of both groups wasirradiated by Er:YAG Iaser with 100～140mJ,0.32cm(fluence of 1250～1750mJ/cm2),150μs,1Hz for 60shots in groupⅠand 120 shots in group Ⅱ,respectively.The eyes were examined with a slit-lamp and enucleated at 3 days,1 month and 4 months postoperativelyfor light microscopy and scanning electron microscopy.Immediately after the Er:YAG laser procedure,whiteyellowish coagulation patches in the ablation area could be seen.In general,th epithelium was repaired com-pletely at approximately 4-8 days postoperatively.Stromal haze could be observed which was most significant at3 to 4 weeks postoperatively.At that time,the epithelium and the underlying stromal collegen fibrils showed hy-perplasia and disorganization.The corneas became clear essentially 4 months postoperatively.All of thesechanges were more significant in group Ⅱ.The possibility to use the Er:YAG Iaser in corneal surgery and its ad-vantages were discussed in this paper.