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RNA干扰技术在药物研究中的应用 被引量：4

Applications of RNA interference technology to drug study
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摘要 RNA干扰是由双链RNA引发的转录后基因沉默。双链RNA经Dicer酶降解成21 23nt的小干扰RNA,并以其为模板,特定位点、特定间隔降解与之序列相应的mRNA。随着RNA干扰机制的深入研究与广泛应用,RNAi已用在药物研究中的各个领域,尤其在药物开发上,能够解决临床前药物开发的一些瓶颈问题,如药靶鉴定,优化药靶,从而节省时间和资金,并提高成功率,加速药物的临床研究。同时RNA在药物研究的其他领域都也显示了卓越的作用,为药物研究提供了强大的工具。 RNA interference is a mechanism of posttranscriptional gene silencing(PTGS) induced by double-stranded RNA. dsRNA is firstly degradated into 21-23 nt siRNA by Dicer RNase, and then makes itself as a template, triggers sequence-specific mRNA to degradate at the specific site and the specific time. Along with the deep study and the wide application of RNAi, RNAi technology can be used to address certain bottlenecks during preclinical drug development.Such technologies currently are being used to help identify the best drug targets for a given disease indication. An emerging and potentially powerful application of these technologies is now being explored to help prioritize drug candidates to lessen cost and time consuming preclinical animal studies. Effective implementation of RNAi technology during preclinical drug development can increase the probability of success and accelerate the path to the clinic. and provide emerging and potentially powerful tools for drug study.
作者 李新平
机构地区 苏州大学生命科学学院生化与分子生物学教研室
出处 《中国药理学通报》 CAS CSCD 北大核心 2005年第4期400-403,共4页 Chinese Pharmacological Bulletin
关键词 RNA干扰技术 药物研究 RNA interference drug study
分类号 R-05 [医药卫生] R394 [医药卫生—医学遗传学]
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参考文献23

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二级参考文献28

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中国药理学通报
2005年 第4期

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