西替利嗪干预组胺诱导的真皮成纤维细胞IL-8和MCP-1产生

The intervention effects of cetirizine on interleukin-8 and monocyte chemotactic protein-1 induced by histamine in dermal fibroblast cells

目的 探讨真皮成纤维细胞组胺H1 受体(histamineH1 receptor,H1R)表达及西替利嗪(cetirizine,CET)对组胺(histamine,HIS)诱导炎症因子的干预作用,寻找CET抗皮肤过敏炎症的作用靶点。方法 采用RT- PCR和免疫组化技术检测真皮成纤维细胞H1RmRNA和蛋白表达;用HIS处理细胞为模型组,ELISA检测HIS诱导IL 8和MCP -1的蛋白分泌及CET的干预作用。结果 真皮成纤维细胞有H1RmRNA和蛋白表达; 10-6 ~10-4 mol·L-1 HIS显著地诱导了真皮成纤维细胞IL -8蛋白分泌(P<0 .05vs对照组),10-5 mol·L-1 HIS明显地诱导了MCP 1分泌(P<0 .01vs对照组),加入10-6、10-5 mol·L-1 CET共同培养24h,抑制了HIS诱导的IL- 8和MCP -1表达(P<0 .05vs模型组)。结论 CET可能通过抑制真皮成纤维细胞趋化因子的表达而发挥抗皮肤过敏炎症作用。

Aim To study the expression of histamine H_1 receptor (H_1R) and the influence of cetirizine (CET) on inflammatory factors induced by histamine (HIS) in dermal fibroblast cells so as to find new action target of anti-allergic inflammation of CET.Methods Expression of H_1R mRNA and protein in dermal fibroblast cells was assessed by means of RT-PCR and immunohistochemistry. The inflammation model was constrcted by HIS. The cells were incubated and treated with HIS in the presence of cetirizine or not. ELISA was used to detect the protein secretion of interleukin-8 and monocyte chemotactic protein-1. Results: Compared with control group, HIS (10^(-6), 10^(-5),10^(-4) mol·L^(-1)) significantly enhanced the protein secretion of interleukin-8 (P<0.05 vs control group). Similarly, HIS (10^(-5) mol·L^(-1)) also significantly enhanced the protein secretion of monocyte chemotactic protein-1 (P<0.01 vs control group). In the presence of CET (10^(-6), 10^(-5) mol·L^(-1)) for 24 h, the histamine-induced effects were inhibited (P<0.05 vs model group).Conclusion CET may exert anti-allergic inflammatory effects on the skin by inhibiting the expression of chemokines in dermal fibroblast cells.