绒毛膜癌的基因表达谱初步研究

Preliminary Study on Gene Expression Profiles of Choriocarcinoma

目的 比较绒毛膜癌与葡萄胎基因表达谱改变, 研究绒毛膜癌的基因表达特征。方法 采用cDNA芯片技术,用Cy3 dUTP标记葡萄胎mRNA, 用Cy5 dUTP标记绒毛膜癌细胞mRNA制备探针进行芯片杂交, 抽取3 条基因作RT PCR验证。结果 从有效基因位点中筛选出227 个差异表达基因,占所研究基因的5.66%, 其中上调基因表达共85 条,占2.12%, 下调基因共142 条,占3.54%。结论 与葡萄胎相比, 绒毛膜癌能引起包括信号转导相关基因、转移相关基因等较广泛的基因激活与上调效应,同时也下调了细胞周期负性调节、蛋白酶抑制因子等相关基因,初步揭示了绒毛膜癌基因调控异常的复杂性。

Objective To explore gene expression profiles of choriocarcinoma to understand mechanisms of malignancy from gene level. Methods The mature cDNA microarray technique was adopted. Fluorescent probes were prepared from RNA labeled with cy3-dUTP for the complete hydatidiform mole groups and with cy5-dUTP for the choriocarcinoma groups through reverse-transcriptation. 3 differential expression genes were analyzed by β-actin semi-quantitative RT-PCR. Results Of the 4004 human genes analyzed by microarray, 227 genes ( 5.66% of the investigated genes) were revealed differential expression in choriocarcinoma groups compared with the complete hydatidiform mole groups, of which 85 genes( 2.12%) were up-regulated and 142 genes( 3.54%) were down-regulated. Conclusion Compared with complete hydatidiform mole, choriocarcinoma cells may up-regulate signal transduction genes, metastasis-related genes, and down-regulate negative modulator of cell cycle, inhibitor of proteases and other related genes. The complexity of choriocarcinoma cells to adjust the gene expression is revealed as a whole by the gene chip technology.