乙醇诱导大鼠急性胃炎后Fos在中枢神经系统的表达

Expression of Fos in central nevrous system after ethanol-induced acute gastritis in rat

目的:探讨乙醇诱导大鼠急性胃炎后Fos在中枢神经系统的表达及其意义。方法:取30只成年雄性Wistar大鼠,随机分为5组,每组6只。A组:单纯生理盐水灌胃组;B组:单纯乙醇灌胃组;C组:膈下迷走神经切断乙醇灌胃组;D组:内脏大、小神经切断乙醇灌胃组;E组:膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。运用免疫组化技术显示Fos阳性神经元在大鼠中枢神经系统(CNS)中的分布。结果:B、C组动物的Fos阳性神经元在丘脑腹后外侧核、第9胸髓后角胶状质内大量存在,疑核和迷走神经背核内有少量聚集;B、D组Fos阳性神经元在疑核和迷走神经背核内有较多聚集,但远低于B、C组在丘脑腹后外侧核、第9胸髓后角胶状质内的Fos阳性神经元数。而A、E组在上述4个核团内Fos阳性神经元则较少。结论:胃的痛觉冲动以交感神经传入为主。

Objective: To study the expression and significance of c-fos in central nervous system after ethanol-induced acute gastritis in rat. Methods: Animals were randomly classified into 5 groups: group A, gaster-poured by physiological solution; group B, gaster-poured by ethanol; group C, gaster-poured by ethanol after hypophrenic vagectomy; group D, gaster-poured by ethanol after big-small splanchnicotomy; group E, gaster-poured by ethanol after hypophrenic vagotomy and big-small splanchnicotomy.The distribution of c-fos positive neuron in central nervous system was displayed and immunohistochemical staining method was used. Results: There were a lot of c-fos-positive neuron in thalamus ventral-lateral nucleus and No 9 thoracic medullaris posterior horn cinerea in groups B and C; Fos- positive neuron in ambiguous nucleus and dorsal nucleus of vagus nerve were better in groups B and D, but they were obiviously less than them of halamus ventral-lateral nucleus and No 9 thoracic medullaris posterior horncinerea in groups B and C. Conclusion: The gastric algesia impulsion are mainly conducted by sympathetic afferent nerve.