白细胞介素-1α基因启动子区域C-889T多态与冠心病的相关性

Correlation between the polymorphism in the promoter region of interleukin-1α gene with the coronary heart disease

目的:研究白细胞介素-1α(interleukin-1α,IL-1α)基因启动子区域-889位C→T多态与冠心病(CH D)的相关性。方法:运用M ALDI-TOF质谱检测技术,对上海地区94例血压正常的CH D患者和100名健康对照者进行C-889T多态基因分型。结果:C-889T多态的基因型分布在CH D组(CC=83、CT=11)和对照组(76、24)间差异有显著性(P=0.026)。C、T等位基因频率与对照组相比差异亦有显著性(94.15%、5.85%/88.00%、22.00%,P=0.035)。在多支病变组中,C-889T多态的基因型分布及其等位基因频率与对照组相比差异均有显著性(46、5/76、24,P=0.036;95.10%、4.90%/88.00%、22.00%,P=0.048)。结论:IL-1α基因启动子区域的C-889T多态可能是血压正常人群CH D发病的一个遗传性危险因素,并且还可能与冠状动脉粥样硬化的病变程度相关。

Objective To analyze the correlation between the C/T polymorphism at position -889 in the promoter region of interleukin-1α (IL-1α) gene and the coronary heart disease (CHD) in Shanghai. Methods MALDI-TOF MassARRAY system was used to genotyping the C-889T polymorphism in 94 normotensive patients with CHD and 100 healthy controls. Results The genotype distribution of the C-889T polymorphism was significantly different between the CHD group (CC=83, CT=11) and the controls (CC=76, CT=24; P=0.026). The frequencies of C and T alleles were also obviously different between the two groups(94.15% and 5.85% vs 88.00% and 22.00%;P=0.035). The genotype distribution and alleles frequencies of the C-889T polymorphism were obviously different between the patients with double-vessel disease (DVD) and triple-vessel disease (TVD) and controls (46 and 5 vs 76 and 24, P=0.036; 95.10% and 4.90% vs 88.00% and 22.00%, P=0.048). Conclusions The C-889T polymorphism in the promoter region of IL-1α gene might be a genetic risk factor for CHD in normotensive patients, which might be also associated with the extent of coronary atherosclerosis.