咪喹莫特对BALB/c小鼠细胞因子生成的影响

Influence of Imiquimod on Cytokine Induction in BALB/c Mice

目的探讨咪喹莫特的免疫调节机制。方法ELISA检测经咪喹莫特灌胃(30mg/kg)后不同时间点的BALB/c小鼠血清干扰素α(IFN-α)、白介素12(IL-12)、干扰素γ(IFN-γ)、白介素4(IL-4)浓度的变化。ELISA检测喂食咪喹莫特的BALB/c小鼠脾细胞在与0.25μg/mL刀豆素A共孵育时分泌IFN-γ、IL-4的能力。结果咪喹莫特体内诱导BALB/c小鼠生成IFN-α、IL-12,在诱导后2h达高峰;咪喹莫特体内无诱导BALB/c小鼠生成IFN-γ、IL-4的作用;接受咪喹莫特灌胃的BALB/c小鼠脾细胞体外受ConA诱导时,分泌IFN-γ和IL-12的能力增强。结论咪喹莫特诱导BALB/c小鼠分泌IFN-α、IL-12等前炎症因子,并由此促进机体获得性免疫。

Objective To study the immunomodulating mechanisms of imiquimod. Methods BALB/c mice were fed with imiquimod(30 mg/kg). After 0.5, 1, 2, 4 hours, serum concentrations of IFN-α, IL-12, IFN-γ, IL-4 were measured by ELISA. The spleen cells of the mice were incubated with 0.25 μg/mL of ConA. IFN-γ and IL-4 were detected by ELISA in the supernatant. Results IL-12 and IFN-α could be induced by imiquimod in vivo. Serum levels of IL-12 and IFN-α peaked in 2 hours after feeding with imiquimod. Serum IFN-γ and IL-4 seemed not to be induced significantly by imiquimod. In vitro, expression of IFN-γ and IL-12 in spleen cells taken from mice fed with imiquimod was enhanced by co-incubation with 0.25 μg/mL ConA. Conclusions Imiquimod could induce the pro-inflammatory cytokine secretion and promote the acquired immunity in BALB/c mice.