尖锐湿疣患者外周血树突细胞亚群的检测

Peripheral Blood Dendritic Cell Subsets in Patients with Condyloma Acuminata

目的检测尖锐湿疣患者外周血树突细胞(DC)亚群并探讨其意义。方法采用免疫荧光三标记流式细胞仪检测25例尖锐湿疣患者外周血DC亚群(DC1/CD11c+和DC2/CD123+),并以26例正常人作为对照。结果尖锐湿疣患者外周血CD123+DC百分数为(0.63±0.34)%比对照组(0.41±0.17)%明显增高(P<0.01)。长病程组及复发患者CD123+DC增高明显(均为P<0.01),而短病程组及初发患者则无明显改变(均为P>0.05)。尖锐湿疣患者组CD11c+DC百分数(1.40%±0.44%)与对照组(1.57%±0.78%)相比差异无统计学意义(P>0.05)。结论尖锐湿疣患者外周血DC2亚群占优势,导致向Th2样免疫反应偏移,此可能与尖锐湿疣的复发和病程的长短有关。

Objective To determine the peripheral blood dendritic cell (DC) subsets in patients with condyloma acuminata (CA) and its significance. Methods The CD11c+ peripheral blood DC (DC1) and CD123+ DC(DC2) subsets were analyzed by flow cytometry with three-color immunofluorescent staining in 25 patients with CA and 26 healthy controls. Results The proportion of CD123+ peripheral blood DCs was significantly increased in patients with CA[(0.63 ± 0.34)%]in comparison with that in healthy controls [(0.41 ± 0.17)%](P < 0.01). It was further found that CD123+ DCs were increased markedly in the patients with long-course of disease [(0.83 ± 0.42)%](P < 0.01) and recurrent groups [(0.74 ± 0.36)%](P < 0.01) respectively, in comparison with patients with short-course of disease [(0.50 ± 0.20)%] and first attack [(0.47 ± 0.24)%](P < 0.05). There was no significant difference of CD11c+ DC proportions between patients [(1.40 ± 0.44)%]and the controls[(1.57 ± 0.78)%](P > 0.05). Conclusions The predominance of CD123+ DCs in peripheral blood of CA may favor Th2-dominated immunity, which may be related to recurrence and course of the disease.