循环抗体和循环抗原检测对泡球蚴病的疗效评价

AVALUATION OF CTRCULATING ANTGEN AND ANTIBODY DETECTION FOR THERAPEUTIC EFFECTS OF SECONDARY CYSTS OF ECHINOCOCCUS MULTILOCULARIS IN MICE

本文对小鼠继发性泡球蚴病用吡喹酮脂质体、吡喹酮、阿苯达唑、甲苯达唑、氟苯达唑进行化疗，对各组治疗期间的小鼠血清用间接血凝（ＩＨＡ）、单克隆抗体（ＭｃＡｂ）、斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ），分别检测循环抗体（ＣＡｂ）和循环抗原（ＣＡｇ）。结果表明，除阿苯达唑组小鼠血清抗体水平在治疗结束时下降明显，与对照组有显著差异外，其余各治疗组的抗体水平下降均不明显。各组治疗结束时，小鼠血清的ＣＡｇ水平明显下降，与对照组有显著差异（Ｐ＜０．０１），表明检测循环抗原可作为疗效考核的指标之一。

In this paper,secondary cysts of Echinococcus multi-locularis in mice were treat-ed with praziquantel-liposome,praziquantel,albendazole,mebendazole and flubendazole.Duringthe treatment period,we continuously evaluated circulating antigen and antibody from mice seraof each treated group by means of IHA,McAb-Dot-ELISA.The results showed that there wereno significant difference in circulating antibody among the different treated groups except alben-dazole group which is significantiy different as compared with control group( P <0.05).The cir-culating antigen was greatly lower at the end of treatment in all treated groups as compared withcontrol group(P<0.01).It means that the circulating antigen can be considered as a indication oftherapeutic effect.