Ⅱ型环氧合酶在膀胱移行细胞癌中的表达及与肿瘤血管发生的关系

Expression of Cyclooxygenase-2 in transitional cell carcinoma of bladder and its relation to tumor angiogenesis

目的:研究Ⅱ型环氧合酶(Cox -2)在膀胱移行细胞癌中的表达,探讨它与膀胱癌病理分级及临床分期的关系及其临床意义,并研究它与血管内皮细胞生长因子(VEGF)及微血管密度(MVD)表达的关系,从而探讨Cox- 2在膀胱肿瘤血管发生中所起的作用。方法:应用免疫组化方法检测Cox- 2、VEGF及MVD在94 例膀胱移行细胞癌、12例膀胱良性病变和5例膀胱癌旁正常组织中的表达。结果:Cox- 2的表达随着膀胱癌分级分期的上升而呈上升趋势(P<0.05);在膀胱癌中,Cox 2 表达与VEGF表达( r=0.716, P=0.000)关系十分密切,与MVD表达存在明显相关性( r=0.458,P=0.000)。结论:Cox- 2在膀胱癌中均为高表达,它的表达参与了肿瘤的发生及恶性进展,并且与膀胱肿瘤新生血管发生有着密切关系。

Objective:To study the expression of Cyclooxygenase-2 in transitional cell carcinonma and the relationship between its expression and the pathological grades and clinical stages of cancer, as well as its clinical significance. In order to evaluate the roles of Cox-2 in the tumor angiogenesis of bladder cancer, the relationship between it and the expression of Vascular Endothelial Growth Factor(VEGF) and Microvessel Density(MVD) were studied. Methods:The expressions of Cox-2, VEGF and MVD were measured by immunohistochemistry in 94 cases of bladder cancer, 12 cases of benign bladder disease and 5 cases of normal tissue beside the tumor. Results:The intensity of Cox-2 expression was increased with the increasing of grades and stages of tumor(P<0.05); The expression of Cox-2 had a very close correlation with that of VEGF(r=0.716,P=0.000), and also correlated with the expression of MVD significantly(r=0.458,P=0.000).Conclusions:Cox-2 has a strong expression in the bladder neoplasms. The expression of it involved in the occurrence and progression of the cancer, as well as in the tumor angiogenesis.