恶性疟原虫FCC1／HN株特异基因片段的克隆及部分序列分析

CLONING AND SEQUENCING OF A DNA FRAGMENT FROM PLASMODIUM FALCIPARUM FCC1/HN ISOLATE

本研究通过分离、培养恶性疟原虫ＦＣＣ１／ＨＮ分离株，提取、纯化其基因组ＤＮＡ，用ＢａｍＨ１部分酶切，并克隆ｐＵＣ１８载体，转化受体菌株大肠杆菌ＪＭ１０３，用基因组ＤＮＡ探针筛选转化子，对杂交信号最强的克隆片段ｐＨＦ１作部分序列分析，在国内首次明确恶性疟原虫ＦＣＣ１／ＨＮ特异ＤＮＡ部分序列。ｐＨＦ１克隆片段约１．２ｋｂ，两端分别测定了３２８和２７１个碱基。Ｇ＋Ｃ含量为２６．８％，并有较多的酶切位点，便于作亚克隆，该序列还可用作ＤＮＡ探针或ＰＣＲ扩增模板有较大实用价值。

A specific DNA fragment isolated from Plasmodium falciparum FCC1/HN isolate has been cloned in Bam H1 site of pUC18 and first partially sequenced by Sangers method.The results show∶G+C percent of DNA sequence is 26.8%,and the cloned DNA has many restriciton sites which are conventional for subcloning.The authors suggest that this clone and sequence may be used as a guide for developing a DNA probe or PCR primers.