阿苯达唑对小鼠肌肉内移行的犬钩虫幼虫的作用

EFFECT OF ALBENDAZOLE ON ANCYLOSTOMA CANINUM LARVAE MIGRATING IN THE MUSCLES OF MICE

小鼠于接种犬钩虫第３期幼虫１０００条后１ｗｋ，ｉｇ阿苯达唑７５、１５０或３００ｍｇ／ｋｇ·ｄ，连给３ｄ时，自各组小鼠肌肉检获的幼虫数为２．７±１．７、２．０±１．５和１．０±１．０条，较对照组的２０５±６８为少。小鼠ｉｇ阿苯达唑１５０ｍｇ／ｋｇ·ｄ×３，并于末次给药后不同时间，用高效液相色谱法测定其血浆和肌肉的阿苯达唑及其有效代谢物阿苯达唑亚砜的含量。结果，在血浆和肌肉中仅查见少量阿苯达唑。但是较高浓度的阿苯达唑亚砜（５．４—１０．５μｇ／ｍｌ）除在血浆中查见外，亦在肌肉中测得（２．２—４．６μｇ／ｇ）。肌肉中的阿苯达唑亚砜含量较高，有益于杀死在肌肉中移行的钩虫幼虫。

When mice inoculated with 1 000 third stage larvae of Ancylostoma caninum for 1 week were treated intragastrically (ig) with albendazole (Alb) 75,150 or 300 mg/kg·d for 3 days,the mean larva numbers collected from the muscles of each group were 2.7 ± 1.7 , 2.0 ± 1.5 and 1.0 ± 1.0 ,respectively,being much less than that 205 ± 68 of the control group.In mice treated ig with Alb 150 mg/ kg ·d for 3 days, the concentrations of Alb and its effective metabolite,albendazole sulfoxide (AlbSO),were determined in plasma and the muscles at different intervals after the last medication using high performance liquid chromatography.The results showed that only low concentrations of Alb were detected in both plasma and the muscles.However,higher concentrations of AlbSO were found not only in the plasma (5.4 10.5 μg/ ml) ,but also in the muscles (2.2 4.6 μg/ g) ,The higher contents of AlbSO in the muscles would be helpful for killing the Ancylostoma larvae migrating in the muscles of mice.