根癌农杆菌介导的叶用莴苣基因转化系统的建立

Establishment of Agrobacterium tumefaciens mediated gene transformation system of leaf lettuce

以叶用莴苣微茎尖为试材,在含有BA和NAA的MS液体培养基上旋转培养,诱导出大量愈伤组织;进一步分化培养,获得大量不定芽并生根;将愈伤组织碎块与根癌农杆菌共培养,对经卡那霉素筛选后所得到的再生植株进行X Gluc染色,蓝色反应阳性率为80%,表明建立了叶用莴苣愈伤组织基因转化系统。

Primordium-like tissue was established from the apical meristem under rotation culture on MS medium with BA and NAA. Adventitious shoots with roots were efficiently induced from the tissue. After the shivered primordium-like tissue were co-cultured with Agrobacterium tumefaciens and the regenerated plants were selected by kanamycin, the regenerated plants showed 80% of positive reaction when their leaf slices dyed with X-Gluc solution. The results showed that the gene transformation system of primordium-like tissue of leaf lettuce was successfully established.