G蛋白β3亚单位基因C825T多态性与高血压患者的肥胖无关

No Association Between G Protein β3 Subunit Gene Polymorphism and Obesity in Hypertensive Patients

目的 探讨G蛋白β3亚单位基因C82 5T多态性与原发性高血压患者肥胖的相关关系。 方法 采用聚合酶链反应结合限制性内切酶片段长度多态分析方法检测 14 7例健康人和 32 1例高血压患者的G蛋白 β3亚单位C82 5T多态性 ,并测定高血压患者的体质指数、总胆固醇、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇及空腹血糖浓度。结果 高血压组G蛋白β3亚单位C82 5T多态性中基因型频率 (CC为 2 8.7% ,CT为 5 2 % ,TT为19.3% )、等位基因频率 (C为 5 4 .6 72 % ,T为 4 5 .33% )与正常对照组基因型频率 (CC为 2 7.2 % ,CT为 4 6 .9% ,TT为 2 5 .9% )、等位基因频率 (C为 5 0 .7% ,T为 4 9.3% )比较无显著性差异 (P >0 .0 5 ) ;CC基因型患者的体质指数、血脂水平与CT +TT基因型患者比较无显著性差异 (P >0 .0 5 )。结论 G蛋白 β3亚单位基因C82

Aim To investigate the relationship between G protein β3 subunit gene polymorphism and obesity in hypertensive patients. Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of G protein of β3 subunit gene in 147 normotensive controls and 321 hypertensive patients. Body mass index was measured. Serum total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, fasting serum glucose were determined in hypertensive patients. Results There were no significant differences of the G protein β3 subunit gene C825T polymorphism genotypes and alleles between hypertensive patients (CC 28.7%, CT 52%, TT 19.3% and C 50.7%, T 49.3%) and normotensive controls (CC 27.2%, CT 46.9%, TT 25.9% and C 50.7%, T 49.3%). Body mass index, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol and fasting serum glucose did not differ significantly between CC and CT+TT genotype in hypertensive patients. Conclusion The C825T polymorphism of G protein β3 subunit was not associated with obesity in hypertensive patients.