yCDgly TK融合自杀基因前药系统对鼻咽癌CNE-2细胞株放射增敏作用的研究

Radiosensitization by yCDglyTK fusion suicide gene/prodrug system in human nasopharyngeal carcinoma CNE-2 cell line

目的研究yCDglyTK融合自杀基因前药系统对人鼻咽癌细胞株CNE2细胞的放射增敏作用。方法pcDNA3.1(-)CMVe·Egr-1yCDglyTK质粒经电穿孔法转染CNE2细胞,给予不同剂量γ射线照射及不同剂量前药,通过免疫组化、高效液相色谱、细胞存活率测定(MTT法)及克隆增殖实验,观察不同剂量辐射对自杀基因阳性CNE-2细胞中自杀基因表达及功能的影响,并观察前药干预及γ射线对CNE-2细胞生长的影响。结果电离辐射可诱导增强自杀基因阳性细胞株中yCDglyTK基因的表达;电离辐射与前药的联合大大增强了对自杀基因阳性CNE-2细胞的杀伤作用,其杀伤作用大于单独自杀基因前药系统和单独放射。结论yCDglyTK融合自杀基因前药系统对CNE-2细胞有放射增敏作用,其与放射联合使用对CNE-2细胞有明显协同杀伤效应。

Objective To investigate the radiosensitization by yCDglyTK fusion suicide gene/prodrug system in human nasopharyngeal carcinoma cells (CNE-2). Methods The plasmid vector of pcDNA3.1(-)CMVe·Egr-1yCDglyTK was introduced into CNE-2 cells by electroporation. Then the cells were exposed to various doses of radiation and prodrug. Observation was made for the change of expression and function of fusion suicide gene in yCDglyTK-expressing cells through immunohistochemistry, HPLC, MTT and clonogenic assay. The influence in growth of CNE-2 cells was observed too. Results Ionization radiation can induce an increased expression of yCDglyTK gene in suicide-gene positive cells; Combined with ionization radiation and prodrug showed greater toxicity to yCDglyTK-expressing CNE-2 cells than disposed with ionization radiation or prodrug alone. Conclusion The yCDglyTK fusion suicide gene/prodrug system can enhance the radiosensitivity of CNE-2 cells, and show synergistic killing effect when combined with ionization radiation.