麦冬总皂甙对培养心肌细胞缺氧再给氧损伤的保护作用

Protective Effect of Ophiopogan Total Saponies on Hy-poxia-Reoxygenation Induced Injury in Cultured Myocar-dial Cells

目的:观察麦冬总皂甙(Ophiopogan Total Saponies,OTS)对培养心肌细胞缺氧再给氧损伤的保护作用。方法:以培养的心肌细胞为模型,在缺氧再给氧损伤细胞后,观察心肌细胞活力及搏动频率的变化,测定培养液上清乳酸脱氢酶的含量。结果:OTS 10 μg/L和40 μg/L可以提高损伤后心肌细胞的活力,提高心肌细胞的搏动频率,并使培养液上清乳酸脱氢酶的含量明显降低。结论:OTS对缺氧再给氧损伤的心肌细胞具有保护作用。

Objective: To explore the effect of Ophiopogan Total Saponies(OTS ) on hypoxia-reoxygenation-induced injury in cultured cardiac myocytes. Method: By using cultured myocardial cells as models, the number of death cells, beating frequency and the content of LDH were observed after hypoxia-ABSTRACTS OF ORIGINAL ARTICLES reoxygenation induced injury. Results: 10 μg/L and 40 μg/L of OTS decreased the number of death cells and the content of LDH, and restored the cellular beating frequency in the supernatant. Conclusion: OTS may protect the cultured myocardial cells against hypoxia-reoxygenation induced injury.