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重组TFPI-2抑制纤溶酶活性的酶促动力学实验方法研究

Kinetics Study on Antiplasmin Activity of Recombinant TFPI-2 in Vitro
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摘要 目的:建立重组TFPI-2抑制纤溶酶活性的测定方法。方法:应用酶促动力学分析方法,建立TFPI-2抑制纤溶酶的测定方法并进行抑制动力学分析。结果:重组TFPI-2对液相纤溶酶均有显抑抑制作用,且呈剂量-效应关系。结论:重组TFPI-2具有强烈抑制纤溶酶活性的作用,为进一步体外探讨TFPI-2在相关领域的应用,提供了可靠的测定方法。 Objective: To establish a assay for plasmin inhibition with the recombi-nant TFPI-2. Method: First investigating suitable conditions for plasmin inhibition assay, then the antiplasmin activities of the recombinant TFPI-2 were tested in fluid. Results: A assay for plasmin inhibition has established, and antiplasmin activity of the recombimant TFPI-2 is confirmed in fluid. Conclusion: Recombinant TFPI-2 has strong antiplasmin activity, which provides the experiment basis for investigating the role of recombinant TFPI-2 in relative fields.
作者 宁勇 黄瑞滨 宋善俊
机构地区 华中科技大学协和医院血液病研究所
出处 《微循环学杂志》 2005年第2期63-66,共4页 Chinese Journal of Microcirculation
基金 国家自然科学基金资助课题 (3 0 2 715 90 )
关键词 力学实验方法 纤溶酶活性 基质金属蛋白酶 组织因子途径抑制物 丝氨酸蛋白酶抑制剂 重组 Factor 肿瘤细胞转移 研究发现 胰蛋白酶 血管生成 基质重塑 血液凝固 细胞凋亡 分子量 Tissue factor pathway inhibitor-2( TFPI-2) Plasmin Enzymatic kinetics analysis Inhibition
分类号 R329 [医药卫生—人体解剖和组织胚胎学] R285.5 [医药卫生—中药学]
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参考文献11

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微循环学杂志
2005年 第2期

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