高效毛细管电泳法测定牛乳铁蛋白的含量

HPCE determination of bovine lactoferrin

目的:采用高效毛细管电泳法(HPCE)的毛细管区带电泳模式(CZE)分离测定牛乳铁蛋白(BLF)的含量.方法:熔融石英毛细管柱67 cm(有效长度55 cm)×50μm;运行缓冲液为33 mmol·L-1磷酸二氢钾-10 mmol·L-1磷酸溶液(pH 2.5);压力进样6.9 kPa × 5 s;运行电压25 kV;检测波长214 nm;毛细管柱温为25℃.结果:乳铁蛋白进样浓度在100.0～600.0 mg·L-1范围内线性关系良好(r=0.999 5),最低检测限为25 mg·L-1.结论:采用HPCE测定乳铁蛋白及杂蛋白的方法简便,结果可靠.

OBJECTIVE To determine the content and the related proteins of bovine lactoferrin (BLF) by HPCE. METHODS Capillary zone electrophoresis (CZE) separation was conducted usihg, fused-silica capillary column: 67 cm (efficiency length 55 cm) 50 μm, running buffer: 33 mmol·L -1 KH_ 2 PO_ 4 -10 mmol·L -1 H_ 3 PO_ 4 solution (pH 2.5 ); eletroinjection: with 6.9 kPa × 5 s; running voltage:25 kV; detection wavelength: at 214 nm; column temperature: at 25 ℃ . RESULTS The calibration curve of BLF was linear in the range of 100.0 - 600.0 mg·L -1 ( r = 0.999 5 ). The detection limit is 25.0 mg L -1 . CONCLUSION The method of determining BLF content and related proteins by HPCE is easy to operate and the results of the determination are accurate.