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酶标仪快速检测Rh-D阴性血型的方法研究

Study on rapid enzyme-reader measure Rh-D negative type of human blood group
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摘要 目的建立一套快速、高效的Rh D阴性血型鉴定方法,用于血站系统大批量Rh D定型实验,以便达到快速、准确、高效检测的目的。方法采用全自动样品处理仪完成对待检红细胞的稀释、分配及加入抗D试剂的全过程。采用酶标仪在620nm波长条件下进行批量比色判读结果。结果利用酶标仪快速检测,在血球稀释浓度为1∶8、Rh检测试剂稀释度为1∶16时,对13800份标本进行检测,结果符合率为100%。结论酶标仪快速检测Rh D阴性血型的方法操作简单、快捷,精确性、重复性较好,对于减少血站系统检验人员工作强度、提高工作质量有较大帮助。 Objective To establish a rapid, accuracy and efficient method for batch sample identification of Rh blood group D-negative.Methods The whole process including dilution of sample erythrocyte, its allocation and adding anti-D agent was treated with full automatic sample processor.The colorimetry of samples was treated with enzymatic-reader at 620 nm.Results The correct rate of Rh blood group D-negetive is 100% for 13 800 blood samples in condition of 1∶8 for erythrocyte dilution and 1∶16 for Rh assay agent dilution.Conclusion This method is simple, rapid for identification of Rh blood group D-negetive with good accuracy and precision, which is quite useful for promoting the work quality and reducing the professional workload in blood station.
出处 《国外医学(临床生物化学与检验学分册)》 2005年第4期199-200,共2页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
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