摘要
目的以大黄酸为指标,进行大黄不同提取物的药动学研究,对大黄工艺优化提供科学依据.方法于大鼠ig大黄不同提取物后不同时间点取血浆,采用LC-MS法建立大黄酸血药浓度的测定方法,经3P87软件计算得药动学参数.结果大鼠血浆中大黄酸在2.0～30μg/mL线性关系良好,ig大黄酸体内药时过程可用二室开放模型描述.结论以AUC为指标,SFE-CO2萃取+树脂精制产物工艺组最佳,SFE-CO 2萃取组次之.
Objective To provide pharmacokinetic evidences for optimization of Rheum palmatum extraction technology, through studying pharmacokinetics of extract by different technologies from R. palmutum with rhein as index. Methods Blood samples were collected at various time-points after ig given different extract to rats. An LC-MS method was established for determination of rhein in rat plasma and pharmacokinetic parameters were calculated by programe 3P87. Results A good linear relationship of rhein existed in the range of 2.0 — 30 μg/mL in rat plasma. The blood concentration-time course of rhein after ig administration can be described as a two-compartment open model. Conclusion Considering the AUC as index, SFE-CO_2 and residue resin purification is the best technology and SFE-CO_2 is the second.
出处
《中草药》
CAS
CSCD
北大核心
2005年第5期687-689,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(39800196)
关键词
大黄
大黄酸
提取工艺
药动学
Rheum palmatum L.
rhein
extraction technology
pharmacokinetics
