摘要
目的建立河北道地药材热河黄芩的HPLC指纹图谱,并与不同产地黄芩药材指纹特征相比较,为科学评价与有效控制黄芩质量提供新方法。方法采用HPLC法测定了热河黄芩等11个不同产地黄芩样品。色谱条件:C18柱,乙腈-0.25%磷酸-四氢呋喃为流动相进行梯度洗脱,检测波长274 nm,体积流量1.0 mL/m in,柱温30℃。结果建立了HPLC指纹图谱共有模式,并对不同产地药材进行了相似度比较。结论色谱指纹图谱分析法能简便、快速地鉴别和区分不同来源的黄芩药材,为全面控制黄芩药材的质量提供了依据。
Objective To set up the HPLC fingerprints of Scutellaria baicalensis Georgi. (SBG) collected from Rehe, Hebei Province and compare the fingerprints of SBG collected from different habitats so as to establish a sensitive and specific method for controlling the quality of SBG. Methods The HPLC fingerprints of SBG from different places were obtained from Agilent 1100 instrument. The HPLC separation was performed on a Diamonsil^(TM) C_(18) analytical column gradient eluted with acetonitrile-0.25% H_3PO_4-THF at the flow rate of 1.0 mL/min. The temperature of column was 30 ℃. The UV detection wavelength was 274 nm. Results The mutual mode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different habitats were compared. Conclusion It is simple and rapid to identify and differentiate SBG collected from different sources with the method which can be used as a quality control item for SBG.
出处
《中草药》
CAS
CSCD
北大核心
2005年第5期743-747,共5页
Chinese Traditional and Herbal Drugs
基金
河北省自然科学基金项目(303453)
