疏血通注射液对局灶性脑缺血大鼠的治疗作用

Therapeutic effect of Shuxuetong injection on focal cerebral ischemia rat

目的:观察疏血通注射液治疗局灶性脑缺血大鼠脑梗死的效果。方法:采用自体血栓注入并闭塞大脑中动脉的方法,制备局灶脑缺血模型。假手术组、大脑中动脉闭塞(MCAO)模型组(模型组)大鼠尾静脉注射生理盐水、疏血通治疗组大鼠尾静脉注射疏血通注射液,均0.25mL·kg-1,qd,连续5d。观察3组大鼠的脑梗死范围、脑含水量、神经功能评分、血管闭塞和再通情况、病理组织学改变等指标的变化。结果:模型组大鼠脑含水量(84±s3)%,比假手术组(74±3)%明显增多;疏血通注射液可明显改善神经功能缺失评分,缩小脑梗死范围,降低脑组织含水量,此外疏血通注射液还可促进血栓溶解,使血管再通并减轻脑组织病理变化。结论:疏血通注射液对局灶性脑缺血大鼠急性脑梗死有明显的治疗作用。

AIM: To study the treatment effect of Shuxuetong injection on a rat model of cerebral infarction. METHODS: A focal cerebral ischemia model of rat was established by embolizing left middle cerebral artery with autologous blood clots. The created models were divided into sham operation group, model group and Shuxuetong group; the former two were injected via tail vein respectively with normal saline, the latter with Shuxuetong injection 0.25 mL·kg^(-1), the dosages contained with same volume as for each and once daily for 5 d. The changes of infarction volume, brain water content, neurologic function scoring, occlusion and recanalization of vessels, as well as histopathological changes were observed for all of the 3 groups. RESULTS: The brain water content in model group was more significantly increased than that in sham group; Shuxuetong injection could improve neurologic dysfunction, decrease the infarction volume, reduce brain water content, accelerate thrombus dissoloving and recanalization of occluded vessels, alleviate histopathological changes remarkably. CONCLUSION: Shuxuetong injection has a significant effect on rats with acute cerebral infarction.