泛素蛋白酶体抑制剂MG-132对胃癌细胞增殖和凋亡的影响

Effects of ubiquitin-proteasome inhibitor MG-132 on proliferation and apoptosis of gastric carcinoma cells

目的:研究泛素蛋白酶体抑制剂对胃癌细胞增殖和凋亡的影响。方法:将泛素蛋白酶体抑制剂MG-132加入胃癌细胞SGC7901,用四氮唑蓝(MTT)法测定细胞生长抑制效应,流式细胞仪检测细胞凋亡,免疫细胞化学检测生存素的表达。结果:MG-132对胃癌细胞有显著的抑制作用,24,48h的IC50分别为80.9,9.1μmol·L-1;MG1325.0μmol·L-1作用胃癌细胞24,48,72,96h后,细胞凋亡率分别为(4.2±s0.6)%,(30±4)%,(47±6)%,(53±6)%,生存素在胃癌细胞中呈高表达,经MG132作用后,表达明显降低。结论:MG132能显著抑制胃癌细胞的增殖、诱导其凋亡,并下调生存素的表达。

AIM: To investigate effects of ubiquitin-proteasome inhibitor on proliferation and apoptosis of gastric carcinoma cells. METHODS: The gastric carcinoma cells SGC-7901 were treated with MG-132. The effects of growth suppression on cells were evaluated with MTT assay. Apoptosis was measured by flow cytometry. Expression of survivin was detected by immunocytochemical technique. RESULTS: MG-132 showed obvious inhibitory effect on the growth of gastric carcinoma cells, IC_(50) of 24 and 48 h were 80.9 and (9.1) μmol·L^(-1) respectively. After treated with (5.0 μmol)·L^(-1) MG-132 for 24, 48, 72 and 96 h, the ratios of apoptotic cells were (4.2±0.6) %, (30±4) %, ((47±6) %) and (53±6) % respectively. Expression of survivin was high in gastric carcinoma cells and it was decreased in cells treated with MG-132. CONCLUSION: MG-132 can significantly inhibit the proliferation of gastric carcinoma cells and induce apoptosis with down ward regulation of survivin expression.