慢性乙型肝炎患者血清HBVDNA含量与IL-8、IL-10水平检测及临床意义

Detection and significance of interlerkin 8 and interleukin 10 levels and HBVDNA in serums from patients with chronic hepatitis B

目的检测慢性乙型肝炎(CHB)患者血清IL-8、IL-10与HBVDNA、ALT水平,探讨乙型肝炎慢性化的机理,为慢性乙型肝炎的治疗提供依据。方法分离72例CHB临床血清标本,用实时荧光定量聚合酶链反应(FQ-PCR)测HBVDNA含量;用双抗体夹心法定量检测血清IL-8、IL-10水平,以16位健康献血者的血清为对照。结果与正常对照相比,CHB患者IL-8、IL-10明显升高,IL-10在HBVDNA阳性组高于HBVDNA阴性组,IL-8在ALT异常组明显高于ALT正常组。结论血清IL-8水平的升高与肝损害程度有关,IL-10水平升高与HBVDNA持续感染有关。

Objective To understand the impacts of interleukin 8(IL-8)、interleukin 10(IL-10) in serums from patients with chronic hepatitis B(CHB) on the persistent infection of hepatitis B virus(HBV),the levls of HBVDNA and IL-8,IL-10 in serums from 72 patients with CHB and 16 normal controls were measured by real time quantitive PCR and ELISA method. The levels of IL-8, IL-10 in serums were greater in patients with CHB than in controls (P<0.01).Patients with seropositive for HBV DNA had higher levels of IL-8,IL-10 in serums than those of patients with seronegative for HBV DNA. The levels of IL-8 were higher in chronic hepatitis B patients with ALT abnormal than those of in patients with ALT normal. Our results suggested that IL-8 could reflect the degree of liver damage in patients with hepatitis B and IL-10 may have an important role in persistent infection of HBV in patient with CHB.