细胞间黏附分子-1基因多态性与老年无症状性脑白质损害的相关性

Association between intercellular adhesion molecule-1 K469E polymorphism and white matter lesions in asymptomatic elderly people

目的 探讨健康老年人无症状性脑白质损害(WML)和细胞间黏附分子1 (ICAM -1)K469E基因多态性之间的关系。方法 利用聚合酶链式反应限制性片段长度多态性法(PCR RFLP),对121例WML患者和99名对照组老年人的ICAM 1K469E基因多态性位点进行检测和分析。结果 病例组和对照组ICAM 1K469E多态性分布符合Hardy Weinberg遗传平衡定律,说明研究对象具有群体代表性。WML组KK基因型和K等位基因频率均明显高于对照组(基因型50. 4%vs25. 3%,P<0. 01;等位基因频率73 .1% vs52. 0%,P=0 .002),两组间差异有统计学意义。Logistic多因素回归分析显示在校正高血压、年龄等因素后,ICAM 1KK型与WML的发生相关(OR=2 99,P=0. 01)。和非KK型个体相比,KK型个体患WML相对危险度是前者的2. 99倍。结论 ICAM 1KK基因型与WML的发生具有相关性,提示ICAM 1基因K469E多态性可能是中国人WML发病的遗传学危险因素。

Objective To evaluate the relationship between intercellular adhesion molecule-1 (ICAM-1) gene K469E polymorphism and white matter lesions (WML) in asymptomatic elderly people.Methods One hundred and twenty-one patients with WML and ninety-nine controls were included in the study. ICAM-1 genotypes were evaluated by polymerse chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results Distributions of each genotype in patient and control groups were in agreement with Hardy-Weinberg equilibrium, indicating that no apparent bias was observed in the populations. The frequency of ICAM-1 KK genotype and K allele were higher in patients with WML than in those without (genotype 50.4% vs 25.3%,P<0.01; allele 73.1% vs 52.0%, P=0.002), the differences between the two groups were significant. In the multiple logistic regression analysis, independent of other risk factors such as hypertension and age, the ICAM-1 KK genotype was associated with a 2.99-fold increased risk for WML.Conclusions We found a significant association between the KK genotype and the presence of WML, these data suggest that the ICAM-1 K469E polymorphism could be a genetic risk factors for WML in asymptomatic elderly people.