青霉素酰化酶基因工程菌大肠杆菌A56（pPA22）的发酵工艺研究

STUDIES ON FERMENTATION TECHNOLOGY OF GENETICENGINEERING STRAIN OF PENICILLIN ACYLASEE. COLI A56(PPA22)

对青霉素酰化酶基因工程菌大肠杆茵Ａ５６（ｐＰＡ２２）作发酵工艺研究。确定了合适的培养条件。找到了替代进口酵母浸出粉的原材料，国产酵母浸出粉完全替代时以３％的用量为好；用价格低廉、货源充沛的玉米浆替代时以５％用量为佳。发酵过程中采用了补料工艺。在４００公升罐中进行试验，培养液的酶活性达到每１００ｍｌ为２２０．４单位，最高罐批为３２０单位。通过不断地茵种选育和发酵工艺改进，在生产罐上４０批平均酶活住达到每１００ｍｌ为４０５．４单位，最高罐批为５４６单位。４０批菌体酶活性每克湿菌体２０４单位，最高罐批达到２７０单位。将这些菌体用于６－ＡＰＡ生产上，酶解了２００余批，６－ＡＰＡ克分子重量收率在８７％左右，效价２６００ｕ／ｍｇ以上，透光度＞４０％。

Studies on the fermentation technology of E. coli A56(p-PA22)were carried out,We have determined the suitable culture condit-ions,e.g.substituting the raw material,the expensive imported yeast extract powder,and established the technology of supplementing nutrition in the coruse of fermentation,In pilot production the enzymatic activity of cultural liquid is 220.4 units and the highiest batch is 320 units,By continuous strain selection and improvement to fermentation technology,the average enzymatic activity of 40 batchCs is increased to 405.4 units and the highiest batch is 546 units in the 6-APA production.