菱角中抗肿瘤活性成分的分离、提纯及结构鉴定

Separation, Purification and Structural Identification of Anti-tumour Compound in Trapa manshurica Fler

采用柱层析分离技术,以硅胶为填料,以石油醚-醋酸乙酯-甲酸(体积比为5∶4∶0.1)为展开剂(Rf=0.4),提取并分离菱角中具有抗肿瘤作用的活性成分,经质谱法(MS)、红外光谱法(IR)及核磁共振法(1HNMR、13CNMR)对分离得到的纯品进行了结构鉴定,确定该化合物为3,4,5-三羟基苯甲酸的二聚体,同时采用四甲基偶氮唑盐(MTT)还原法、光学显微镜及透射电子显微镜等方法对其抗癌机理进行初步探讨,实验结果表明,该化合物具有诱导肿瘤细胞凋亡的抗肿瘤作用.

The anti-tumour compound in Trapa manshurica Fler was separated and purified by using the silica gel column chromatography, with an eluting agent of petroleum ether-ethyl acetate-formic acid(volume ratio is 5∶4∶0.1, R_f=0.4). The structure of compound Ⅰ obtained was identified to be 3,4,5-trihydroxybenzoic acid dimmer by means of MS, IR, ()~1H NMR and ()^(13)C NMR, and the preliminary study shows that it has a quite high activity for inhibiting the proliferation of hela cell induction apoptosis to anti-tumor mechanism by means of MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction method, optical microscope, transmission electron microscope and flow cytometry.