p120^(ctn)在非小细胞肺癌组织中的表达及其临床意义

Expression of p120^(ctn) and its signific ance in non-small cell lung cancer

目的 探讨p120ctn在非小细胞肺癌组织中的表达及与各临床病理因素和预后的关系。方法 应用p120ctn单克隆抗体,采用免疫组织化学SP法检测143例非小细胞肺癌p120ctn表达情况,其中36例同时应用Westernblot方法对其蛋白表达和亚型情况进行了观察。结果 免疫组织化学:正常支气管黏膜细胞p120ctn表达为细胞膜强阳性,而在非小细胞肺癌中绝大多数表现为膜表达减弱或出现胞质内异位表达,其异常表达率为79 .7% ( 114 /143 )。异常表达与组织学类型无关(P>0 .05),但与肿瘤分化程度、TNM分期和淋巴结转移相关(P<0 .05),与非小细胞肺癌患者不良预后正相关。胞质的异位表达更容易出现在胞膜表达减弱的病例中,鳞癌中胞质异位表达高于腺癌。Westernblot:正常组织中p120ctn总蛋白量明显高于非小细胞肺癌,二者差异有统计学意义(P=0 .003)。正常肺组织p120ctn以亚型1(相对分子质量120 000)和亚型3(相对分子质量100 000)表达为主,而肿瘤组织中则以亚型1表达缺失或减弱为主。结论 p120ctn在非小细胞肺癌组织有异常表达,可作为评估预后的辅助指标。

Objective To investigate the expression of p120 ctn in non-small cell lun g cancer (NSCLC) and its correlation with the clinical and pathologic parameters . Methods Immunohistochemistry (S-P method) was used to detect the expression of p120 ctn in 143 NSCLC cases. The variation of protein expression was fu rther analyzed in 36 cases by Western blot. The correlation with clinical and p athologic parameters was studied. Results Immunohistochemically, normal bronchial cells showed membranous expre ssion for p120 ctn, while NSCLC was characterized by cytoplasmic or diminis hed membranous staining. The rate of abnormal p120 ctn expression was 79. 7% (114/143). There was a significant correlation between abnormal expression o f p120 ctn and tumor differentiation, clinical stage, lymph node metastasis and poor prognosis (P< 0.05), but not histologic typing. Western blot sho wed that the total amount of p120 ctn in normal bronchial cells was signifi cantly higher than that in NSCLC. The p120 ctn isoform 1 (120 000) and iso form 3 (100 000) were expressed in normal lung tissue, while there was a reduced expression or absence of isoform 1 in NSCLC. Conclusion The expression of p120 ctn is abnormal in NSCLC; p120 ctn m ay serve as a useful prognostic marker for NSCLC.