两种免疫缺陷动物人胃癌原位移植模型的肿瘤生物学特性研究

Biological Properties of Orthotopic Implant Models for Human Gastric Carcinoma in Nude and SCID Mice

目的探讨裸小鼠和SCID小鼠两种免疫缺陷动物人胃癌原位移植后肿瘤生长和转移等生物学特性的差异。方法将MKN-45细胞株接种至裸小鼠皮下,成瘤后采用组织学完整的组织块移植于裸小鼠和SCID小鼠胃壁建立原位移植模型,观察所建模型的原位成瘤率、移植瘤生长、侵袭和转移情况。结果①两种免疫缺陷动物的原位成瘤率都为100%;②裸小鼠原位移植瘤平均体积2884±1337mm3,腹腔淋巴结、肝、肺、膈转移率分别为67%、83%、33%和8%;③SCID小鼠原位移植瘤平均体积4582±1326 mm3(P<0.05),肝转移率90%(P>0.05),与裸小鼠较为接近, 腹腔淋巴结转移率90%,肺和膈转移率分别为100%(P<0.01)和60%(P<0.05)。结论证明应用T、B细胞联合免疫缺陷的SCID小鼠较裸小鼠更适用于建立胃癌的原位移植模型。

Objective To explore differential biological properties of local and metastatic human gastric cancer after orthotopic implantation in nude mice and SCID mice. Methods MKN-45 human gastric carcinoma cell line was inoculated subcutaneously in nude mice to develop s.c. growing tumors. Orthotopic implant model simulating human gastric cancer was established by suturing histologically intact human tumor tissue on the stomach walls of nude mice and SCID mice. Subsequently, we observed the take rate of the orthotopic tumors and their biological properties of tumor growth and metastasis. Results ① 100% tumor take rates were observed in both strains of mice.② The tumor volume in the stomach of nude mice was 2884±1337 mm3, the metastases of local and distant lymph nodes were noted in 67% of the mice, the rates of liver, lung and diaphragm metastases were 83%, 33%, 8%, respectively.③ The tumor volume in the stomach of SCID mice was 4582 ±1326 mm3(P<0.05).The incidences of liver metastases in SCID mice were almost similar to those of nude mice which were 90% (P>0.05). In contrast, there was a significant increase in the incidences of lung and diaphragm metastases, which were 100% (P<0.01) and 60% (P<0.05), respectively. Conclusions The results suggest that SCID mice are more suitable for establishment of orthotopic implant models for human gastric carcinoma compared with nude mice.