摘要
目的建立有大量蛋白尿的慢性肾衰动物模型。方法先用5/6肾切除法制作慢性肾功能衰竭的大鼠模型,成功后再分别给予不同蛋白质含量饲料喂养2月,制作有大量蛋白尿的慢性肾衰动物模型。结果正常组大鼠的血肌酐、24 h尿蛋白定量显著低于不同蛋白质含量饲料喂养的模型大鼠(P<0.05),普通饲料+40%蛋白质饲料喂养的模型大鼠血肌酐、24 h尿蛋白定量显著高于普通饲料+20%蛋白质饲料组及普通饲料组,肾脏病理学观察显示:40%高蛋白质饲料组病理改变较其余各组严重,间质纤维化及肾小球硬化程度均较严重,有大量蛋白质管型。结论通过给予5/6肾切除大鼠不同蛋白含量饲料,可以建立有大量蛋白质尿的慢性肾衰动物模型,为临床研究蛋白尿加重慢性肾衰的机制提供了一种新型的实验动物模型。
Objective: To establish Chronic Renal Failure (CRF) with high-grade proteinuria rat model. Methods: The 5/6 nephrectomy rat was replicated and fed with high-protein diet. Results: Volume of Scr and 24h proteinuria in CRF rat's with 40% high-protein diet group were increased significantly than those of normal diet control and 20% high-protein diet group. Renal pathology showed more severely neprosclerosis and matrix fibrosis in 40% high-protein diet group than other groups. Conclusion: CRF with high-grade proteinuria rat models can be established by supplying high-protein diet to replicated 5/6 nephrectomy rats.
出处
《上海实验动物科学》
2005年第1期17-20,33,共5页
Shanghai Laboratory Animal Science
基金
上海市科委资助课题(01DJ19013)
