兔髂动脉再狭窄模型的建立

Establishment of Iliac Arterial Re-stenotic Model in Rabbit

目的建立兔骼动物再狭窄模型。方法通过Fogarty导管经隐动脉入路髂动脉内皮一次损伤和经股动脉入路球囊扩张二次损伤建立兔髂动脉再狭窄模型。应用下肢动脉造影、电镜学和病理学检查研究术后即刻至84 d共9个时段病变动脉狭窄情况。结果手术成功的28条肢体右髂动脉狭窄率>50%-80%,6条肢体右髂动脉狭窄率<50%。新生内膜和中膜以血管平滑肌细胞(VSMCs)为主,随术后时间延长,VSMCs减少而泡沫细胞增多;细胞成分减少,胶原成分增多;新生内膜比例增大,中膜比例减小。结论改进的兔髂动脉再狭窄模型稳定可靠,手术方法简便可行。

Objective To establish rabbit iliac arterial re-stenotic models. Methods Endothelium-denudation in iliac artery was cunducted by Fogarly catheter and second injury by balloon angioplasty, and thirty-six New Zealand White rabbits were used in the experiment. Saphenous artery was accessed lor the first injury, and femoral artery for the second injury. Arteriography, electroscopic and pathological examinations were used to detect re-stenotic samples excised immediately and to day 84 after balloon angioplasty. Results Among the 34 limbs which were successfully done, re-stenotic rate was 50%-80% in 28 right iliac arteries, and less than 50% in 6. Main components of neointima and media were vascular smooth muscle cells (VSMCs). With the progress of the time after operation, numbers of VSMCs were decreased while the foam cells were increased, cellular components were decreased while the collagen components were increased, proportion of neo-intima was increased while that of media was decreased. Conclusion The improved rabbit re-stenotic models were stable and reliable, and the surgical procedures were easily as well.