术前希罗达化疗对大肠癌组织TP/PD-ECGF表达和血管生成的影响

Effect of preoperative chemotherapy on expression of TP/PD-ECGF and angiogenesis in colon cancer tissues

目的研究术前希罗达化疗对大肠癌胸苷磷酸化酶血小板衍化内皮细胞生长因子(TP/PD-ECGF)和肿瘤组织微血管密度(MVD)的影响。方法对30例术前应用希罗达化疗的大肠癌和30例术前未化疗的大肠癌组织,采用免疫组织化学检测TP/PD-ECGF表达及微血管密度。结果术前化疗组TP PD ECGF表达阳性率为35.0%,对照组为63.0%;化疗组和对照组MVD值分别为37.4±18.0和65.8±12.5,差异有显著性。结论术前化疗能使TP PD ECGF活性降低,从而抑制大肠癌的肿瘤血管生成。

Objective To study the effect of preoperative chemotherapy with 5-DFUR on the expression of TP/PD-ECGF (thymidine phosphorylase/platelet-derived endothelial cell growth factor) and MVD (microvessel density) in colon cancer tissues.Methods Thirty cases receiving preoperative 5-DFUR chemotherapy and 30 cases without preoperative chemotherapy were analyzed.Immunohistochemical staining was performed using CD34^+ and TP/PD-ECGF monoclonal antibodies to detect MVD and TP/PD-ECGF expression.Results The positive expression rate of TP/PD-ECGF was (35.0%) and 63.0% in chemotherapy group and control group respectively. MVD was 37.4±18.0 in chemotherapy group and 65.8±12.5 in control group.There was statistical significance in expression of TP/PD-ECGF and MVD between two groups.Conclusion Preoperative chemotherapy can decrease the activity of TP/PD-DCGF and inhibit the angiogenesis of colon cancer.