CRP对人脐静脉内皮细胞释放ET、PAI-1的影响

Effect of CRP on releasing ET,PAI-1 in human umbilical vein endothelial cells

目的:探讨CRP对人脐静脉内皮细胞(HUVEC)释放内皮素-1(ET-1)、纤溶酶原激活物抑制剂-1(PAI-1)的影响。方法:用不同浓度的C反应蛋白(CRP)与HUVEC培养不同时间后,用放射免疫法分析及用ELISA法检测培养液中ET-1、PAI-1的含量。结果:用CRP作用HUVEC后,ET-1、PAI-1的浓度随CRP浓度增加和CRP作用时间延长而呈递增趋势。结论:CRP可促进HUVEC释放ET-1、PAI-1,从而促使血管痉挛、血栓形成和急性冠脉综合征的发生发展。

objective:To observe the effect of CRP on secretions of ET-1、PAI- 1 in human umbilical vein endothelial cells (HUVEC). Medthods: Incubation of HUVEC with different concentration of CRP for indifferent time, and then measure the concentration of ET-1,PAI-1 in cultural fluid by ELLISA and radioimmunoassay. Results:Incubation of HUVEC with CRP results in a time- and dosw-dependent increase in secreted ET-1、PAI-1. Conclusions: CRP induces the production of ET-1、PAI-1 in HUVEC and thus has implications for angiospasm 、 thrombosis and ACS.