南瓜属4个栽培种种子蛋白质电泳分析

Analysis of Electrophoretic Pattern of Seed Protein of Four Cultivated Species in Cucurbita

通过聚丙烯酰胺凝胶电泳(PAGE)对南瓜属4个栽培种中国南瓜、印度南瓜、美洲南瓜及黑籽南瓜的12个品种种子蛋白组分进行了分析。结果显示,黑籽南瓜种子以盐溶蛋白条带居多;其它3个栽培种以水溶蛋白条带居多,其次为盐溶蛋白;4个栽培种酸溶蛋白条带均较少;醇溶蛋白未分离得到蛋白谱带。水溶、盐溶、酸溶蛋白电泳图谱显示各栽培种间蛋白谱带差异明显,同一栽培种品种间蛋白谱带较为一致。

Using polyacrylamide gel electrophoresis(PAGE) to anlysis seed protein ingredients of 12 cultivars from four cultivated species in genus Cucurbita: C. moschato Duch, C. maxima Duch, C. pepo L., C. ficifolia. The results showed: Among seed proteins extracted with water, 0.5 mol·L^(-1) NaCl,0.1 mol·L^(-1) Lacetic acid and 70% Ethanol, most of them are salt-soluble proteins in C. ficifolia; In the other three cultivated species,most of them are water-soluble proteins,with little salt-soluble proteins; All of the four species have very little acid-soluble proteins;In the examination,no alcohol-soluble protein bandings were obtained.The maps of water-soluble proteins, salt-soluble proteins, acid-soluble proteins showed great difference among the four species,but there were almost no difference among the cultivars of the same specie.