陆英中乌索酸和齐墩果酸动态含量的研究

Study on Content of Ursolic Acid and Oleanolic Acid in Sambucus chinensis

建立了反相高效液相色谱法测定不同采收期陆英叶、茎、根中乌索酸和齐墩果酸含量的方法。色谱柱为SymmetryShieldRP18柱(3.9mm×150mm,5μm);流动相为乙腈∶水∶磷酸(体积比,65∶35∶0.1);流速为1.0ml/min;二极管阵列检测器检测,检测波长210nm;柱温25℃。乌索酸线性范围0.096～2.304μg,线性回归系数大于0.9999,检出限为0.3mg/L(3σ);齐墩果酸线性范围0.098～2.352μg,线性回归系数大于0.9997,检出限为0.2mg/L(3σ)。样品中乌索酸的平均回收率为100.3%,RSD为1.5%;齐墩果酸的平均回收率为98.9%,RSD为1.2%(n=5)。该方法简便,准确,线性范围宽,重现性好,适于对含有该成分的药物和药材进行质量评价。

A determination method of ursolic acid and oleanolic acid in leaves, stems and roots of Sambucus chinensis in various collecting periods by reversed-phase high performance liquid chromtograph was established. The chromatographic column, Symmetry Shield RP18(3.9 mm×150 mm,5 μm), ethanenitrile-water-phosphoric acid (65∶35∶0.1, V / V / V) mobile phase with the flow rate of 1.0 ml / min, the detected wavelength (210 nm) and the column temperature(25 ℃) were adopted. The calibration curve of ursolic acid was linear under the content of 0.096～2.304 μg, the correlation coefficient was over 0.999 9, the detected limit was 0.3 mg / L(3σ). The calibration curve of oleanolic acid was linear under the content of 0.098～2.352 μg, the correlation coefficient was over 0.999 7, the detected limit was 0.2 mg / L(3σ).The average recovery of ursolic acid was 100.03 % and RSD was 1.5 %(n=5). The average recovery of oleanolic acid was 98.9 % and RSD was 1.2 %(n=5). The method was simple,accurate and had wider linear range. The method can be adapted to evaluate the quality of medicinal plants that contained ursolic acid and oleanolic acid.