钙—茜素S络合物体系在汞膜电极上的电化学行为及其应用

STUDY AND APPLICATION ON THE ELECTROCHEMICAL BEHAVIOR OF CALSIUM-ARS AT A MERCURY FILM ELECTRODE

在0.1 mol KOH介质中,用微分脉冲伏安法在汞膜玻碳电极上得到钙-茜素S络合吸附波,峰电位为-1.17V(vs.Ag-AgCl),峰电流与钙的浓度在5×10-8～4.2×10-5mol·L-1范围内呈良好的线性关系,检测限为2×10-8 mol·L-1.此络合波应用于血清中微量钙的测定,得到满意的结果.实验表明,该波为1:2的钙-茜素S的络合吸附波,还原过程为伴随两电子参与的不可逆电极反应.

With a mercury film electrode, in 0.1 mol·L -1 KOH and 4.5×10 -4 mol·L -1 ARS solution,a sensitive complex absorptive wave of Ca-ARS is obtained by differential pulse voltammetry. The peak potential is -1.17 V(vs.Ag-AgCl) . The peak current is proportional to the concentration of calcium in the range of 5.0× 10 -8 ~ 4.2×10 -5 mol·L -1 with a detection limit of 2×10 -8 mol·L -1 .This method can be applied successfully to the trace determination of calcium in blood serum. The electrochemical behavior of the system is also studied by cyclic voltammetry, and the experiment results show that the electrode process is irreversible with absorption in the presentation of two electrons.