青藤碱、环孢素A对大鼠外周血单个核细胞的协同抑制作用

Synergistic effects of sinomenine in combination with the immunosuppressive drug cyclosporine A on peripheral blood mononuclear cells of rats

目的探讨青藤碱(sinomenine)对大鼠外周血单个核细胞的作用,进而研究其抑制作用与凋亡的关系。方法采用M TT比色及磷脂酰丝氨酸外翻分析(Annexin V)法。结果青藤碱能够显著的抑制刀豆蛋白A(ConA)和混合淋巴细胞(MLC)激活的单个核细胞增殖,随着浓度的增加其抑制作用加强,环孢素A(CsA)能够显著地抑制ConA激活单个核细胞增殖,加入低剂量的青藤碱后其抑制作用明显增加,青藤碱不能诱导单个核细胞的凋亡。结论青藤碱通过抑制单个核细胞增殖来发挥免疫抑制作用,且与CsA有协同作用。

Objective To investigate the in vitro effects of sinomenine on rat lymphocyte then and to find out the relationship between the suppressive effects and apoptosis. Methods MTT colorimetry and Annexin V were used. Results sinomenine can suppress the proliferation of lymphocyte actived by ConA and MLC, with the combined use of both drugs, a very strong synergistic interaction was produced when compared with monotherapy of CsA. but sinomenine can not lead to apoptosis of lymphocyte. Conclusions the immunosuppressive effects of sinomenine is due to suppressive effect of proliferation of lymphocyte and sinomenine has synergistic effects with CsA .