特异性和非特异性荧光偏振免疫法测定全血环孢素的比较

COMPARISON BETWEEN SELECTIVE AND NONSELECTIVE TDx CYCLOSPORIN ASSAYS

对２０位肾移植术后病人的４１份环孢素（ｃｙｃｌｏｓｐｏｒｉｎ）血样，用美国Ａｂｂｏｔｔ公司的荧光偏振免疫分析法即单克隆全血环孢素测定法（ＳＴＤｘ）和全血环孢素及其代谢物测定法（ＮＳＴＤｘ）进行测定，所得资料用统计学ｔ检验和线性回归分析进行处理。结果显示：服药后８ｈ和１２ｈ体内环孢素原型率有非常显著差异（Ｐ＜０．００１），它们分别为４６％（８ｈ）和３０％（１２ｈ）。在两方法测定结果之间有较好相关性（ｒ＝０．９９，１２ｈ和ｒ＝０．９７，８ｈ）。我们认为，特异性单克隆全血环孢素法能较好地确定体内原型药物浓度，有利于揭示环孢素本身的作用；而非特异性的全血环孢素法能够反映原型药物和主要代谢物的综合作用，由于它们之间存在较好的相关性，可经通过某些换算，来估价和比较它们的治疗浓度范围。

Forty three whole blood samples from 20 patients who had recieved cyclosporin after renal transpiantation were monitored with cyclosporin monoclonal whole blood TDx assay and cyclosporin and metabolites whole blood TDx assay.The results were handled with t test and linear regression analysis There was a good linear relationship between these selsctive and no-selective TDx assavs.8 hours and 12 hours after adminstration,the correlation coefficients were 0.97 and 0.99 respectively.The ratios of selective assay to no-selective assay varied significantly from 8 to 12 hours after administration(P<0.001).The percentage of original cyclosporin in the blood at these two points were 46% and 30% respectively.We concluded that selective TDx assay was good at revealing the effect of cyclosporin itself,and no-selective TDx assay represented the effect of both cyclosporin and it's metabolites.